Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers

Asmaa Abdelrahman, Alaa S. Gouda, Per T. Jørgensen, Jesper Wengel*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This is the first report exploring the capability of twisted intercalating nucleic acid (TINA) and naphthalene-functionalized non-nucleosidic linkers to stabilize and engage in double-helical structures. Four designs were studied with respect to the formation of duplexes and/or other types of self-assemblies. One of the constructs involving TINA provides a thermostable duplex. The biophysical properties of the individual constructs were investigated by UV thermal melting experiments, circular dichroism, and fluorescence emission spectroscopy. Molecular modeling studies were performed in attempts of explaining the biophysical measurements for the duplex based on the TINA-containing oligonucleotide strands.

Original languageEnglish
JournalNucleosides, Nucleotides and Nucleic Acids
ISSN1525-7770
DOIs
Publication statusE-pub ahead of print - 1. Nov 2019

Fingerprint

Oligonucleotides
Nucleic Acids
Monomers
Molecular modeling
Fluorescence spectroscopy
Emission spectroscopy
Circular Dichroism
Self assembly
Freezing
Melting
Experiments
naphthalene
Hot Temperature

Keywords

  • DNA
  • naphthalene
  • pi-interactions
  • pyrene
  • supramolecule

Cite this

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title = "Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers",
abstract = "This is the first report exploring the capability of twisted intercalating nucleic acid (TINA) and naphthalene-functionalized non-nucleosidic linkers to stabilize and engage in double-helical structures. Four designs were studied with respect to the formation of duplexes and/or other types of self-assemblies. One of the constructs involving TINA provides a thermostable duplex. The biophysical properties of the individual constructs were investigated by UV thermal melting experiments, circular dichroism, and fluorescence emission spectroscopy. Molecular modeling studies were performed in attempts of explaining the biophysical measurements for the duplex based on the TINA-containing oligonucleotide strands.",
keywords = "DNA, naphthalene, pi-interactions, pyrene, supramolecule",
author = "Asmaa Abdelrahman and Gouda, {Alaa S.} and J{\o}rgensen, {Per T.} and Jesper Wengel",
year = "2019",
month = "11",
day = "1",
doi = "10.1080/15257770.2019.1683188",
language = "English",
journal = "Nucleosides, Nucleotides and Nucleic Acids",
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Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers. / Abdelrahman, Asmaa; Gouda, Alaa S.; Jørgensen, Per T.; Wengel, Jesper.

In: Nucleosides, Nucleotides and Nucleic Acids, 01.11.2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers

AU - Abdelrahman, Asmaa

AU - Gouda, Alaa S.

AU - Jørgensen, Per T.

AU - Wengel, Jesper

PY - 2019/11/1

Y1 - 2019/11/1

N2 - This is the first report exploring the capability of twisted intercalating nucleic acid (TINA) and naphthalene-functionalized non-nucleosidic linkers to stabilize and engage in double-helical structures. Four designs were studied with respect to the formation of duplexes and/or other types of self-assemblies. One of the constructs involving TINA provides a thermostable duplex. The biophysical properties of the individual constructs were investigated by UV thermal melting experiments, circular dichroism, and fluorescence emission spectroscopy. Molecular modeling studies were performed in attempts of explaining the biophysical measurements for the duplex based on the TINA-containing oligonucleotide strands.

AB - This is the first report exploring the capability of twisted intercalating nucleic acid (TINA) and naphthalene-functionalized non-nucleosidic linkers to stabilize and engage in double-helical structures. Four designs were studied with respect to the formation of duplexes and/or other types of self-assemblies. One of the constructs involving TINA provides a thermostable duplex. The biophysical properties of the individual constructs were investigated by UV thermal melting experiments, circular dichroism, and fluorescence emission spectroscopy. Molecular modeling studies were performed in attempts of explaining the biophysical measurements for the duplex based on the TINA-containing oligonucleotide strands.

KW - DNA

KW - naphthalene

KW - pi-interactions

KW - pyrene

KW - supramolecule

U2 - 10.1080/15257770.2019.1683188

DO - 10.1080/15257770.2019.1683188

M3 - Journal article

C2 - 31674270

AN - SCOPUS:85074729583

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

SN - 1525-7770

ER -