Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers

Asmaa Abdelrahman, Alaa S. Gouda, Per T. Jørgensen, Jesper Wengel*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

This is the first report exploring the capability of twisted intercalating nucleic acid (TINA) and naphthalene-functionalized non-nucleosidic linkers to stabilize and engage in double-helical structures. Four designs were studied with respect to the formation of duplexes and/or other types of self-assemblies. One of the constructs involving TINA provides a thermostable duplex. The biophysical properties of the individual constructs were investigated by UV thermal melting experiments, circular dichroism, and fluorescence emission spectroscopy. Molecular modeling studies were performed in attempts of explaining the biophysical measurements for the duplex based on the TINA-containing oligonucleotide strands.

Original languageEnglish
JournalNucleosides, Nucleotides and Nucleic Acids
Volume39
Issue number1-3
Pages (from-to)82-96
ISSN1525-7770
DOIs
Publication statusPublished - 2020

Keywords

  • DNA
  • naphthalene
  • pi-interactions
  • pyrene
  • supramolecule

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