Non-covalent encapsulation of siRNA with cell-penetrating peptides

Martina Tuttolomondo*, Henrik J. Ditzel*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review


SiRNAs may act as selective and potent therapeutics, but poor deliverability in vivo is a limitation. Among the recently proposed vectors, cell-penetrating peptides (CPPs), also referred as protein transduction domains (PTDs), allow siRNA stabilization and increased cellular uptake. This chapter aims to guide scientists in the preparation and characterization of CPP-siRNA complexes, particularly the evaluation of novel CPPs variants for siRNA encapsulation and delivery. Herein, we present a collection of methods to determine CPP-siRNA interaction, encapsulation, stability, conformation, transfection, and silencing efficiency.

Original languageEnglish
Title of host publicationDesign and Delivery of SiRNA Therapeutics
EditorsHenrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen
PublisherHumana Press
Publication date2021
ISBN (Print)978-1-0716-1297-2
ISBN (Electronic)978-1-0716-1298-9
Publication statusPublished - 2021
SeriesMethods in Molecular Biology

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.


  • Cell-penetrating peptides
  • Knockdown
  • Protein transduction domains
  • RNA interference
  • Short interfering RNA
  • Silencing
  • Small interfering RNA


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