Non-covalent encapsulation of siRNA with cell-penetrating peptides

Martina Tuttolomondo; Henrik J. Ditzel

doi:10.1007/978-1-0716-1298-9_19

Non-covalent encapsulation of siRNA with cell-penetrating peptides

Martina Tuttolomondo^*, Henrik J. Ditzel^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

SiRNAs may act as selective and potent therapeutics, but poor deliverability in vivo is a limitation. Among the recently proposed vectors, cell-penetrating peptides (CPPs), also referred as protein transduction domains (PTDs), allow siRNA stabilization and increased cellular uptake. This chapter aims to guide scientists in the preparation and characterization of CPP-siRNA complexes, particularly the evaluation of novel CPPs variants for siRNA encapsulation and delivery. Herein, we present a collection of methods to determine CPP-siRNA interaction, encapsulation, stability, conformation, transfection, and silencing efficiency.

Original language	English
Title of host publication	Design and Delivery of SiRNA Therapeutics
Editors	Henrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen
Volume	2282
Publisher	Humana Press
Publication date	2021
Pages	353-376
ISBN (Print)	978-1-0716-1297-2
ISBN (Electronic)	978-1-0716-1298-9
DOIs	https://doi.org/10.1007/978-1-0716-1298-9_19
Publication status	Published - 2021

Series	Methods in Molecular Biology
Volume	2282
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.

Keywords

Cell-penetrating peptides
Knockdown
Protein transduction domains
RNA interference
Short interfering RNA
Silencing
Small interfering RNA

Documents & Links

10.1007/978-1-0716-1298-9_19

Cite this

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abstract = "SiRNAs may act as selective and potent therapeutics, but poor deliverability in vivo is a limitation. Among the recently proposed vectors, cell-penetrating peptides (CPPs), also referred as protein transduction domains (PTDs), allow siRNA stabilization and increased cellular uptake. This chapter aims to guide scientists in the preparation and characterization of CPP-siRNA complexes, particularly the evaluation of novel CPPs variants for siRNA encapsulation and delivery. Herein, we present a collection of methods to determine CPP-siRNA interaction, encapsulation, stability, conformation, transfection, and silencing efficiency.",

keywords = "Cell-penetrating peptides, Knockdown, Protein transduction domains, RNA interference, Short interfering RNA, Silencing, Small interfering RNA",

author = "Martina Tuttolomondo and Ditzel, {Henrik J.}",

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Non-covalent encapsulation of siRNA with cell-penetrating peptides. / Tuttolomondo, Martina ; Ditzel, Henrik J.
Design and Delivery of SiRNA Therapeutics. ed. / Henrik J. Ditzel; Martina Tuttolomondo; Sakari Kauppinen. Vol. 2282 Humana Press, 2021. p. 353-376 (Methods in Molecular Biology, Vol. 2282).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

TY - CHAP

T1 - Non-covalent encapsulation of siRNA with cell-penetrating peptides

AU - Tuttolomondo, Martina

AU - Ditzel, Henrik J.

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2021.

PY - 2021

Y1 - 2021

N2 - SiRNAs may act as selective and potent therapeutics, but poor deliverability in vivo is a limitation. Among the recently proposed vectors, cell-penetrating peptides (CPPs), also referred as protein transduction domains (PTDs), allow siRNA stabilization and increased cellular uptake. This chapter aims to guide scientists in the preparation and characterization of CPP-siRNA complexes, particularly the evaluation of novel CPPs variants for siRNA encapsulation and delivery. Herein, we present a collection of methods to determine CPP-siRNA interaction, encapsulation, stability, conformation, transfection, and silencing efficiency.

AB - SiRNAs may act as selective and potent therapeutics, but poor deliverability in vivo is a limitation. Among the recently proposed vectors, cell-penetrating peptides (CPPs), also referred as protein transduction domains (PTDs), allow siRNA stabilization and increased cellular uptake. This chapter aims to guide scientists in the preparation and characterization of CPP-siRNA complexes, particularly the evaluation of novel CPPs variants for siRNA encapsulation and delivery. Herein, we present a collection of methods to determine CPP-siRNA interaction, encapsulation, stability, conformation, transfection, and silencing efficiency.

KW - Cell-penetrating peptides

KW - Knockdown

KW - Protein transduction domains

KW - RNA interference

KW - Short interfering RNA

KW - Silencing

KW - Small interfering RNA

U2 - 10.1007/978-1-0716-1298-9_19

DO - 10.1007/978-1-0716-1298-9_19

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AN - SCOPUS:85105159236

SN - 978-1-0716-1297-2

VL - 2282

T3 - Methods in Molecular Biology

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BT - Design and Delivery of SiRNA Therapeutics

A2 - Ditzel, Henrik J.

A2 - Tuttolomondo, Martina

A2 - Kauppinen, Sakari

PB - Humana Press

ER -

Non-covalent encapsulation of siRNA with cell-penetrating peptides

Abstract

Bibliographical note

Keywords

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