Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

Jan Homolak; Ivan Kodvanj; Ana Babic Perhoc; Davor Virag; Ana Knezovic; Jelena Osmanovic Barilar; Peter Riederer; Melita Salkovic-Petrisic

doi:10.1016/j.mex.2021.101611

Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

Jan Homolak^*, Ivan Kodvanj, Ana Babic Perhoc, Davor Virag, Ana Knezovic, Jelena Osmanovic Barilar, Peter Riederer, Melita Salkovic-Petrisic

^*Corresponding author for this work

KI, OUH, Research unit of Psychiatry (Odense)

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations. • NRP is a rapid, cost-effective, and simple method for reductive capacity assessment • NRP is compatible with a high-throughput screening of solid and liquid biological samples • HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis

Original language	English
Article number	101611
Journal	MethodsX
Volume	9
ISSN	2215-0161
DOIs	https://doi.org/10.1016/j.mex.2021.101611
Publication status	Published - Jan 2022

Bibliographical note

Publisher Copyright:
© 2021 The Authors

Keywords

Antioxidant
Nitrocellulose
Nitrocellulose Redox Permanganometry (NRP)
Oxidative stress
Potassium permanganate
Reductive capacity
ROS

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10.1016/j.mex.2021.101611Licence: CC BY

Open Access VersionFinal published version, 1.07 MBLicence: CC BY

Cite this

@article{28681b4b383c4a738bf8fff771b15649,

title = "Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment",

abstract = "We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations. • NRP is a rapid, cost-effective, and simple method for reductive capacity assessment • NRP is compatible with a high-throughput screening of solid and liquid biological samples • HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis",

keywords = "Antioxidant, Nitrocellulose, Nitrocellulose Redox Permanganometry (NRP), Oxidative stress, Potassium permanganate, Reductive capacity, ROS",

author = "Jan Homolak and Ivan Kodvanj and {Babic Perhoc}, Ana and Davor Virag and Ana Knezovic and {Osmanovic Barilar}, Jelena and Peter Riederer and Melita Salkovic-Petrisic",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2022",

month = jan,

doi = "10.1016/j.mex.2021.101611",

language = "English",

volume = "9",

journal = "MethodsX",

issn = "2215-0161",

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TY - JOUR

T1 - Nitrocellulose redox permanganometry

T2 - A simple method for reductive capacity assessment

AU - Homolak, Jan

AU - Kodvanj, Ivan

AU - Babic Perhoc, Ana

AU - Virag, Davor

AU - Knezovic, Ana

AU - Osmanovic Barilar, Jelena

AU - Riederer, Peter

AU - Salkovic-Petrisic, Melita

PY - 2022/1

Y1 - 2022/1

N2 - We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations. • NRP is a rapid, cost-effective, and simple method for reductive capacity assessment • NRP is compatible with a high-throughput screening of solid and liquid biological samples • HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis

AB - We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations. • NRP is a rapid, cost-effective, and simple method for reductive capacity assessment • NRP is compatible with a high-throughput screening of solid and liquid biological samples • HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis

KW - Antioxidant

KW - Nitrocellulose

KW - Nitrocellulose Redox Permanganometry (NRP)

KW - Oxidative stress

KW - Potassium permanganate

KW - Reductive capacity

KW - ROS

U2 - 10.1016/j.mex.2021.101611

DO - 10.1016/j.mex.2021.101611

M3 - Journal article

C2 - 35004232

AN - SCOPUS:85121609911

SN - 2215-0161

VL - 9

JO - MethodsX

JF - MethodsX

M1 - 101611

ER -

Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

Abstract

Bibliographical note

Keywords

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