Here we tested the neuroprotective effects of crude methanolic extracts of R. rosea roots (Clone 5, Pharmaplant, Germany, grown for four years) as well as chemical fractions and/or purified compounds (e.g. salidrosid, rosavin) against excitotoxicity and ischemia-like brain damage using organotypic hippocampal slice cultures.
Hippocampal slice cultures derived from 8 days old rat pups were grown for 2-3 weeks before exposure to N-methyl-D-aspartate (NMDA, 10 µM, 24 h) or oxygen-glucose deprivation (OGD, 30 or 35 min), with and without presence of R. rosea extracts or compounds during and 24 h after the insult. NMDA- or OGD-induced neuronal cell death was monitored and quantified by propidium iodide uptake and immunohistochemical staining for MAP2 as a neuronal marker.
Significant and dose-dependent protection against NMDA and OGD-induced CA1 pyramidal cell death was obtained by crude extracts using 250 µg/ml (33-50% protection) or 500 µg/ml (45-65% protection). A number of chemical fractions of methanolic Rhodiola extracts, as well as the purified constituents salidrosid and rosavin were tested, but - so far - none of the tested fractions or single constituents showed protection against NMDA or OGD. To study the mechanisms of action of R. rosea extracts, we are currently performing micro array microRNA and gene analyses of treated cultures.
Supported by the Danish Strategic Research Council.
|Publication date||16. Jul 2012|
|Number of pages||1|
|Publication status||Published - 16. Jul 2012|
|Event||8th FENS Forum of Neuroscience - Barcelona, Spain|
Duration: 14. Jul 2012 → 18. Jul 2012
|Conference||8th FENS Forum of Neuroscience|
|Period||14/07/2012 → 18/07/2012|