Natural RNA circles function as efficient microRNA sponges

Thomas B Hansen, Trine I Jensen, Bettina H Clausen, Jesper Bertram Bramsen, Bente Finsen, Christian Kroun Damgaard, Jørgen Kjems

Research output: Contribution to journalLetterResearchpeer-review

Abstract

MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that act by direct base pairing to target sites within untranslated regions of messenger RNAs. Recently, miRNA activity has been shown to be affected by the presence of miRNA sponge transcripts, the so-called competing endogenous RNA in humans and target mimicry in plants. We previously identified a highly expressed circular RNA (circRNA) in human and mouse brain. Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner. Although the circRNA is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets. In the mouse brain, we observe overlapping co-expression of ciRS-7 and miR-7, particularly in neocortical and hippocampal neurons, suggesting a high degree of endogenous interaction. We further show that the testis-specific circRNA, sex-determining region Y (Sry), serves as a miR-138 sponge, suggesting that miRNA sponge effects achieved by circRNA formation are a general phenomenon. This study serves as the first, to our knowledge, functional analysis of a naturally expressed circRNA.
Original languageEnglish
JournalNature
Volume495
Issue number7441
Pages (from-to)384-8
ISSN0028-0836
DOIs
Publication statusPublished - 21. Mar 2013

Fingerprint

Porifera
MicroRNAs
RNA
Argonaute Proteins
Base Pairing
Neurons
Messenger RNA

Keywords

  • Animals
  • Argonaute Proteins
  • Brain
  • Gene Expression Regulation
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs
  • RNA
  • Sex-Determining Region Y Protein

Cite this

Hansen, T. B., Jensen, T. I., Clausen, B. H., Bramsen, J. B., Finsen, B., Damgaard, C. K., & Kjems, J. (2013). Natural RNA circles function as efficient microRNA sponges. Nature, 495(7441), 384-8. https://doi.org/10.1038/nature11993
Hansen, Thomas B ; Jensen, Trine I ; Clausen, Bettina H ; Bramsen, Jesper Bertram ; Finsen, Bente ; Damgaard, Christian Kroun ; Kjems, Jørgen. / Natural RNA circles function as efficient microRNA sponges. In: Nature. 2013 ; Vol. 495, No. 7441. pp. 384-8.
@article{a68ed822852e46e99654ad60ca157ddb,
title = "Natural RNA circles function as efficient microRNA sponges",
abstract = "MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that act by direct base pairing to target sites within untranslated regions of messenger RNAs. Recently, miRNA activity has been shown to be affected by the presence of miRNA sponge transcripts, the so-called competing endogenous RNA in humans and target mimicry in plants. We previously identified a highly expressed circular RNA (circRNA) in human and mouse brain. Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner. Although the circRNA is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets. In the mouse brain, we observe overlapping co-expression of ciRS-7 and miR-7, particularly in neocortical and hippocampal neurons, suggesting a high degree of endogenous interaction. We further show that the testis-specific circRNA, sex-determining region Y (Sry), serves as a miR-138 sponge, suggesting that miRNA sponge effects achieved by circRNA formation are a general phenomenon. This study serves as the first, to our knowledge, functional analysis of a naturally expressed circRNA.",
keywords = "Animals, Argonaute Proteins, Brain, Gene Expression Regulation, HEK293 Cells, HeLa Cells, Humans, Male, Mice, Mice, Inbred C57BL, MicroRNAs, RNA, Sex-Determining Region Y Protein",
author = "Hansen, {Thomas B} and Jensen, {Trine I} and Clausen, {Bettina H} and Bramsen, {Jesper Bertram} and Bente Finsen and Damgaard, {Christian Kroun} and J{\o}rgen Kjems",
year = "2013",
month = "3",
day = "21",
doi = "10.1038/nature11993",
language = "English",
volume = "495",
pages = "384--8",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7441",

}

Hansen, TB, Jensen, TI, Clausen, BH, Bramsen, JB, Finsen, B, Damgaard, CK & Kjems, J 2013, 'Natural RNA circles function as efficient microRNA sponges', Nature, vol. 495, no. 7441, pp. 384-8. https://doi.org/10.1038/nature11993

Natural RNA circles function as efficient microRNA sponges. / Hansen, Thomas B; Jensen, Trine I; Clausen, Bettina H; Bramsen, Jesper Bertram; Finsen, Bente; Damgaard, Christian Kroun; Kjems, Jørgen.

In: Nature, Vol. 495, No. 7441, 21.03.2013, p. 384-8.

Research output: Contribution to journalLetterResearchpeer-review

TY - JOUR

T1 - Natural RNA circles function as efficient microRNA sponges

AU - Hansen, Thomas B

AU - Jensen, Trine I

AU - Clausen, Bettina H

AU - Bramsen, Jesper Bertram

AU - Finsen, Bente

AU - Damgaard, Christian Kroun

AU - Kjems, Jørgen

PY - 2013/3/21

Y1 - 2013/3/21

N2 - MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that act by direct base pairing to target sites within untranslated regions of messenger RNAs. Recently, miRNA activity has been shown to be affected by the presence of miRNA sponge transcripts, the so-called competing endogenous RNA in humans and target mimicry in plants. We previously identified a highly expressed circular RNA (circRNA) in human and mouse brain. Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner. Although the circRNA is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets. In the mouse brain, we observe overlapping co-expression of ciRS-7 and miR-7, particularly in neocortical and hippocampal neurons, suggesting a high degree of endogenous interaction. We further show that the testis-specific circRNA, sex-determining region Y (Sry), serves as a miR-138 sponge, suggesting that miRNA sponge effects achieved by circRNA formation are a general phenomenon. This study serves as the first, to our knowledge, functional analysis of a naturally expressed circRNA.

AB - MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that act by direct base pairing to target sites within untranslated regions of messenger RNAs. Recently, miRNA activity has been shown to be affected by the presence of miRNA sponge transcripts, the so-called competing endogenous RNA in humans and target mimicry in plants. We previously identified a highly expressed circular RNA (circRNA) in human and mouse brain. Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner. Although the circRNA is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets. In the mouse brain, we observe overlapping co-expression of ciRS-7 and miR-7, particularly in neocortical and hippocampal neurons, suggesting a high degree of endogenous interaction. We further show that the testis-specific circRNA, sex-determining region Y (Sry), serves as a miR-138 sponge, suggesting that miRNA sponge effects achieved by circRNA formation are a general phenomenon. This study serves as the first, to our knowledge, functional analysis of a naturally expressed circRNA.

KW - Animals

KW - Argonaute Proteins

KW - Brain

KW - Gene Expression Regulation

KW - HEK293 Cells

KW - HeLa Cells

KW - Humans

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - MicroRNAs

KW - RNA

KW - Sex-Determining Region Y Protein

U2 - 10.1038/nature11993

DO - 10.1038/nature11993

M3 - Letter

VL - 495

SP - 384

EP - 388

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7441

ER -

Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK et al. Natural RNA circles function as efficient microRNA sponges. Nature. 2013 Mar 21;495(7441):384-8. https://doi.org/10.1038/nature11993