Nanoparticle embedded enzymes for improved lateral flow sensors

Veli Cengiz Özalp, Uğur S. Zeydanlı, Anita Lunding, Murat Kavruk, M. Tufan Öz, Füsun Eyidoğan, Lars Folke Olsen, Hüseyin A. Öktem

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In this study, combining the nanoparticle embedded sensors with lateral flow assays, a novel strategy for ensuring the quality of signalling in lateral flow assays (LFAs) was developed. A LFA for reactive oxygen species (ROS) is reported that is based on horse radish peroxidase (HRP) which is co-entrapped with Texas Red dextran inside porous polyacrylamide nanoparticles. In this system, enzymes are protected in the porous matrix of polyacrylamide which freely allows the diffusion of the analyte. The sensor is rapid and sensitive for quantification of hydrogen peroxide concentrations. A test solution of hydrogen peroxides was quantified with this novel LFA-ROS sensor to obtain a linear range between 1 and 25 μM. Nanoparticle embedding of enzymes is proposed here as a general strategy for developing enzyme-based lateral flow assays, eliminating adverse effects associated with biological samples.
Original languageEnglish
JournalAnalyst
Volume138
Pages (from-to)4255-4359
Number of pages5
ISSN0003-2654
DOIs
Publication statusPublished - 2013

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