Multilayer diffusion modeling and coherent anti-Stokes Raman scattering microscopy for spatially resolved water diffusion measurements in human skin

Irina Iachina, Michael A. Lomholt, Johannes H. Eriksen, Jonathan R. Brewer*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In this work using Coherent anti-Stokes Raman Scattering microscopy, it was possible to directly measure the time dependent, spatially resolved change in concentration of water (D2O) in intact skin tissue with a spatial resolution of under 1 μm, and combined with a multilayer diffusion model, diffusion coefficients at different depths in the tissue were extracted. The results show that the diffusion varies at different layers throughout the Stratum Corneum (SC), indicating that the SC is not a homogeneous barrier but a complicated heterogeneous structure. Interestingly, averaging over the diffusion at the different depths and samples gave a relatively constant value of 0.047 ± 0.01 μm2/second. Treating the skin with acetone or tape stripping led to an increased diffusion coefficient of 0.064 ± 0.02 μm2/second and 0.079 ± 0.03 μm2/second, respectively. The combined method and model presented here shows potential for wide applications for measuring spatially resolved diffusion of different substances in a variety of different samples.

Original languageEnglish
Article numbere202200110
Journaljournal of biophotonics
Volume15
Issue number10
Number of pages10
ISSN1864-063X
DOIs
Publication statusPublished - Oct 2022

Keywords

  • CARS
  • measurements
  • multilayer diffusion modeling
  • skin Barrier
  • water diffusion
  • Microscopy
  • Humans
  • Skin/diagnostic imaging
  • Water/chemistry
  • Spectrum Analysis, Raman/methods
  • Acetone

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