Modulation of i-motif thermodynamic stability by the introduction of UNA (unlocked nucleic acid) monomers

Anna Pasternak, Jesper Wengel

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The influence of acyclic RNA derivatives, UNA (unlocked nucleic acid) monomers, on i-DNA thermodynamic stability has been investigated. The 22 nt human telomeric fragment was chosen as the model sequence for stability studies. UNA monomers modulate i-motif stability in a position-depending manner. The largest destabilization is observed for position C14, while UNA placed in position A12 causes significant increase of i-DNA thermodynamic stability. CD curves of UNA-modified variants imply no structural changes relative to the native i-motif.
Original languageEnglish
JournalBioorganic & Medicinal Chemistry Letters
Volume21
Issue number2
Pages (from-to)752–755
Number of pages4
ISSN0960-894X
DOIs
Publication statusPublished - 2011

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Nucleic Acids
Thermodynamic stability
Monomers
Modulation
compound A 12
DNA
RNA
Derivatives

Cite this

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title = "Modulation of i-motif thermodynamic stability by the introduction of UNA (unlocked nucleic acid) monomers",
abstract = "The influence of acyclic RNA derivatives, UNA (unlocked nucleic acid) monomers, on i-DNA thermodynamic stability has been investigated. The 22 nt human telomeric fragment was chosen as the model sequence for stability studies. UNA monomers modulate i-motif stability in a position-depending manner. The largest destabilization is observed for position C14, while UNA placed in position A12 causes significant increase of i-DNA thermodynamic stability. CD curves of UNA-modified variants imply no structural changes relative to the native i-motif.",
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journal = "Bioorganic & Medicinal Chemistry Letters",
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Modulation of i-motif thermodynamic stability by the introduction of UNA (unlocked nucleic acid) monomers. / Pasternak, Anna; Wengel, Jesper.

In: Bioorganic & Medicinal Chemistry Letters, Vol. 21, No. 2, 2011, p. 752–755.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Modulation of i-motif thermodynamic stability by the introduction of UNA (unlocked nucleic acid) monomers

AU - Pasternak, Anna

AU - Wengel, Jesper

PY - 2011

Y1 - 2011

N2 - The influence of acyclic RNA derivatives, UNA (unlocked nucleic acid) monomers, on i-DNA thermodynamic stability has been investigated. The 22 nt human telomeric fragment was chosen as the model sequence for stability studies. UNA monomers modulate i-motif stability in a position-depending manner. The largest destabilization is observed for position C14, while UNA placed in position A12 causes significant increase of i-DNA thermodynamic stability. CD curves of UNA-modified variants imply no structural changes relative to the native i-motif.

AB - The influence of acyclic RNA derivatives, UNA (unlocked nucleic acid) monomers, on i-DNA thermodynamic stability has been investigated. The 22 nt human telomeric fragment was chosen as the model sequence for stability studies. UNA monomers modulate i-motif stability in a position-depending manner. The largest destabilization is observed for position C14, while UNA placed in position A12 causes significant increase of i-DNA thermodynamic stability. CD curves of UNA-modified variants imply no structural changes relative to the native i-motif.

U2 - 10.1016/j.bmcl.2010.11.106

DO - 10.1016/j.bmcl.2010.11.106

M3 - Journal article

VL - 21

SP - 752

EP - 755

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

SN - 0960-894X

IS - 2

ER -