MMP Mediated Degradation of Type IV Collagen Alpha 1 and Alpha 3 Chains Reflects Basement Membrane Remodeling in Experimental and Clinical Fibrosis - Validation of Two Novel Biomarker Assays

Jannie Marie Sand, Lise Skakkebæk Larsen, Cory Hogaboam, Fernando Martinez, Meilan Han, Martin Røssel Larsen, Arkadiusz Nawrocki, Qinlong Zheng, Morten Asser Karsdal, Diana Julie Leeming

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide fragments (neo-epitopes) into the circulation. Here we present the development of two competitive enzyme-linked immunosorbent assays (ELISAs) for assessing the levels of specific fragments of type IV collagen α1 (C4M12a1) and α3 (C4M12a3) chains in serum as indicators of fibrosis.
Original languageEnglish
JournalPLOS ONE
Volume8
Issue number12
Pages (from-to)e84934
ISSN1932-6203
DOIs
Publication statusPublished - 2013

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