TY - JOUR
T1 - MMP Mediated Degradation of Type IV Collagen Alpha 1 and Alpha 3 Chains Reflects Basement Membrane Remodeling in Experimental and Clinical Fibrosis - Validation of Two Novel Biomarker Assays
AU - Sand, Jannie Marie
AU - Larsen, Lise Skakkebæk
AU - Hogaboam, Cory
AU - Martinez, Fernando
AU - Han, Meilan
AU - Larsen, Martin Røssel
AU - Nawrocki, Arkadiusz
AU - Zheng, Qinlong
AU - Asser Karsdal, Morten
AU - Leeming, Diana Julie
PY - 2013
Y1 - 2013
N2 - Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide fragments (neo-epitopes) into the circulation. Here we present the development of two competitive enzyme-linked immunosorbent assays (ELISAs) for assessing the levels of specific fragments of type IV collagen α1 (C4M12a1) and α3 (C4M12a3) chains in serum as indicators of fibrosis.
AB - Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide fragments (neo-epitopes) into the circulation. Here we present the development of two competitive enzyme-linked immunosorbent assays (ELISAs) for assessing the levels of specific fragments of type IV collagen α1 (C4M12a1) and α3 (C4M12a3) chains in serum as indicators of fibrosis.
U2 - 10.1371/journal.pone.0084934
DO - 10.1371/journal.pone.0084934
M3 - Journal article
C2 - 24376856
VL - 8
SP - e84934
JO - P L o S One
JF - P L o S One
SN - 1932-6203
IS - 12
ER -