Microfluidic culture chamber for the long-term perfusion and precise chemical stimulation of organotypic brain tissue slices

H. H. Caicedo*, M. Vignes, B. Brugg, J. M. Peyrin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

We have developed a microfluidic perfusion-based culture system to study long-term in-vitro responses of organo-typic brain slices exposed to localized neurochemical stimulation. Using this microperfusion chamber we show that hip-pocampal organotypic brain slices cultures grown on nitrocellulose membranes can be stimulated for up to 24 hours in our experimental setup while preserving tissue viability. By using fluorescent probe we show that a specific area of the hippocampal slice can be precisely targeted and stimulated. The device allows a virtual pixelisation of slice, precise control of the in-vitro micro environment, long-term culture of viable brain slices, and delivery of fluids to selected brain regions in a multiplexed and spatially defined manner.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Number of pages3
Volume3
Publication date1. Dec 2010
Pages1835-1837
ISBN (Print)9781618390622
Publication statusPublished - 1. Dec 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 3. Oct 20107. Oct 2010

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period03/10/201007/10/2010

Fingerprint

Perfusion
Collodion
Fluorescent Dyes
Hip
Equipment and Supplies
Membranes
In Vitro Techniques

Keywords

  • Localized neurochemical stimulation
  • Long-term perfusion
  • Microfluidics
  • Organotypic brain slice

Cite this

Caicedo, H. H., Vignes, M., Brugg, B., & Peyrin, J. M. (2010). Microfluidic culture chamber for the long-term perfusion and precise chemical stimulation of organotypic brain tissue slices. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 3, pp. 1835-1837)
Caicedo, H. H. ; Vignes, M. ; Brugg, B. ; Peyrin, J. M. / Microfluidic culture chamber for the long-term perfusion and precise chemical stimulation of organotypic brain tissue slices. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. pp. 1835-1837
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Caicedo, HH, Vignes, M, Brugg, B & Peyrin, JM 2010, Microfluidic culture chamber for the long-term perfusion and precise chemical stimulation of organotypic brain tissue slices. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 3, pp. 1835-1837, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 03/10/2010.

Microfluidic culture chamber for the long-term perfusion and precise chemical stimulation of organotypic brain tissue slices. / Caicedo, H. H.; Vignes, M.; Brugg, B.; Peyrin, J. M.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. p. 1835-1837.

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

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Caicedo HH, Vignes M, Brugg B, Peyrin JM. Microfluidic culture chamber for the long-term perfusion and precise chemical stimulation of organotypic brain tissue slices. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3. 2010. p. 1835-1837