Methylation of geometrically constrained lysine analogues by histone lysine methyltransferases

Abbas H K Al Temimi, Paul B White, Marcus J M Mulders, Nicole G A van der Linden, Richard H Blaauw, Anita Wegert, Floris P J T Rutjes*, Jasmin Mecinović*

*Corresponding author for this work

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Abstract

We report synthesis and enzymatic assays on human histone lysine methyltransferase catalysed methylation of histones that possess lysine and its geometrically constrained analogues containing rigid (E)-alkene (KE), (Z)-alkene (KZ) and alkyne (Kyne) moieties. Methyltransferases G9a and GLP do have a capacity to catalyse methylation in the order K ≫ KE > KZ ∼ Kyne, whereas monomethyltransferase SETD8 catalyses only methylation of K and KE.

Original languageEnglish
JournalChemical Communications
Volume56
Issue number20
Pages (from-to)3039-3042
ISSN1359-7345
DOIs
Publication statusPublished - 2020

Keywords

  • Alkenes/chemistry
  • Alkynes/chemistry
  • Biocatalysis
  • Histone-Lysine N-Methyltransferase/metabolism
  • Humans
  • Lysine/analogs & derivatives
  • Methylation
  • Molecular Conformation

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