Meta-analysis of Chromatin Programming by Steroid Receptors

Ville Paakinaho, Erin E. Swinstead, Diego M. Presman, Lars Grøntved, Gordon L. Hager*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

39 Downloads (Pure)

Abstract

Transcription factors (TFs) must access chromatin to bind to their response elements and regulate gene expression. A widely accepted model proposes that only a special subset of TFs, pioneer factors, can associate with condensed chromatin and initiate chromatin opening. We previously reported that steroid receptors (SRs), not considered pioneer factors, can assist the binding of an archetypal pioneer, the forkhead box protein 1 (FOXA1), at a subset of receptor-activated enhancers. These findings have been challenged recently, with the suggestion that newly acquired data fully support the prevailing pioneer model. Here, we reexamine our results and confirm the original conclusions. We also analyze and discuss a number of available datasets relevant to chromatin penetration by SRs and find a general consensus supporting our original observations. Hence, we propose that chromatin opening at some sites can be initiated by SRs, with a parallel recruitment of factors often treated as having a unique pioneer function. This Matters Arising paper is in response to Glont et al. (2019), published in Cell Reports.

Original languageEnglish
JournalCell Reports
Volume28
Issue number13
Pages (from-to)3523-3534
Number of pages15
ISSN2211-1247
DOIs
Publication statusPublished - 24. Sep 2019

Fingerprint

Steroid Receptors
Chromatin
Meta-Analysis
Transcription Factors
Forkhead Transcription Factors
Response Elements
Gene expression

Keywords

  • chromatin
  • chromatin remodelers
  • dynamic assisted loading
  • FOXA1
  • pioneer factors
  • steroid receptors
  • transcription factors

Cite this

Paakinaho, V., Swinstead, E. E., Presman, D. M., Grøntved, L., & Hager, G. L. (2019). Meta-analysis of Chromatin Programming by Steroid Receptors. Cell Reports, 28(13), 3523-3534. https://doi.org/10.1016/j.celrep.2019.08.039
Paakinaho, Ville ; Swinstead, Erin E. ; Presman, Diego M. ; Grøntved, Lars ; Hager, Gordon L. / Meta-analysis of Chromatin Programming by Steroid Receptors. In: Cell Reports. 2019 ; Vol. 28, No. 13. pp. 3523-3534.
@article{96fd2862f7a54b4f8f4ca46ebeb6cdce,
title = "Meta-analysis of Chromatin Programming by Steroid Receptors",
abstract = "Transcription factors (TFs) must access chromatin to bind to their response elements and regulate gene expression. A widely accepted model proposes that only a special subset of TFs, pioneer factors, can associate with condensed chromatin and initiate chromatin opening. We previously reported that steroid receptors (SRs), not considered pioneer factors, can assist the binding of an archetypal pioneer, the forkhead box protein 1 (FOXA1), at a subset of receptor-activated enhancers. These findings have been challenged recently, with the suggestion that newly acquired data fully support the prevailing pioneer model. Here, we reexamine our results and confirm the original conclusions. We also analyze and discuss a number of available datasets relevant to chromatin penetration by SRs and find a general consensus supporting our original observations. Hence, we propose that chromatin opening at some sites can be initiated by SRs, with a parallel recruitment of factors often treated as having a unique pioneer function. This Matters Arising paper is in response to Glont et al. (2019), published in Cell Reports.",
keywords = "chromatin, chromatin remodelers, dynamic assisted loading, FOXA1, pioneer factors, steroid receptors, transcription factors",
author = "Ville Paakinaho and Swinstead, {Erin E.} and Presman, {Diego M.} and Lars Gr{\o}ntved and Hager, {Gordon L.}",
year = "2019",
month = "9",
day = "24",
doi = "10.1016/j.celrep.2019.08.039",
language = "English",
volume = "28",
pages = "3523--3534",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "13",

}

Paakinaho, V, Swinstead, EE, Presman, DM, Grøntved, L & Hager, GL 2019, 'Meta-analysis of Chromatin Programming by Steroid Receptors', Cell Reports, vol. 28, no. 13, pp. 3523-3534. https://doi.org/10.1016/j.celrep.2019.08.039

Meta-analysis of Chromatin Programming by Steroid Receptors. / Paakinaho, Ville; Swinstead, Erin E.; Presman, Diego M.; Grøntved, Lars; Hager, Gordon L.

In: Cell Reports, Vol. 28, No. 13, 24.09.2019, p. 3523-3534.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Meta-analysis of Chromatin Programming by Steroid Receptors

AU - Paakinaho, Ville

AU - Swinstead, Erin E.

AU - Presman, Diego M.

AU - Grøntved, Lars

AU - Hager, Gordon L.

PY - 2019/9/24

Y1 - 2019/9/24

N2 - Transcription factors (TFs) must access chromatin to bind to their response elements and regulate gene expression. A widely accepted model proposes that only a special subset of TFs, pioneer factors, can associate with condensed chromatin and initiate chromatin opening. We previously reported that steroid receptors (SRs), not considered pioneer factors, can assist the binding of an archetypal pioneer, the forkhead box protein 1 (FOXA1), at a subset of receptor-activated enhancers. These findings have been challenged recently, with the suggestion that newly acquired data fully support the prevailing pioneer model. Here, we reexamine our results and confirm the original conclusions. We also analyze and discuss a number of available datasets relevant to chromatin penetration by SRs and find a general consensus supporting our original observations. Hence, we propose that chromatin opening at some sites can be initiated by SRs, with a parallel recruitment of factors often treated as having a unique pioneer function. This Matters Arising paper is in response to Glont et al. (2019), published in Cell Reports.

AB - Transcription factors (TFs) must access chromatin to bind to their response elements and regulate gene expression. A widely accepted model proposes that only a special subset of TFs, pioneer factors, can associate with condensed chromatin and initiate chromatin opening. We previously reported that steroid receptors (SRs), not considered pioneer factors, can assist the binding of an archetypal pioneer, the forkhead box protein 1 (FOXA1), at a subset of receptor-activated enhancers. These findings have been challenged recently, with the suggestion that newly acquired data fully support the prevailing pioneer model. Here, we reexamine our results and confirm the original conclusions. We also analyze and discuss a number of available datasets relevant to chromatin penetration by SRs and find a general consensus supporting our original observations. Hence, we propose that chromatin opening at some sites can be initiated by SRs, with a parallel recruitment of factors often treated as having a unique pioneer function. This Matters Arising paper is in response to Glont et al. (2019), published in Cell Reports.

KW - chromatin

KW - chromatin remodelers

KW - dynamic assisted loading

KW - FOXA1

KW - pioneer factors

KW - steroid receptors

KW - transcription factors

U2 - 10.1016/j.celrep.2019.08.039

DO - 10.1016/j.celrep.2019.08.039

M3 - Journal article

C2 - 31553919

AN - SCOPUS:85072257433

VL - 28

SP - 3523

EP - 3534

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 13

ER -