Measurement of total CO2 in microliter samples of urine and other biological fluids using infrared detection of CO2

Francesco Trepiccione, Francesco Maria Iena, Laura Catalini, Francesco Martino Carpi, Mogens Koed, Sebastian Frische*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The purpose of this study is to describe a low-cost and simply made instrument capable of measuring the total CO 2 content of microliter volumes of biological fluids utilizing a commercially available CO 2 sensor based on a NDIR detector. The described instrument is based on transformation of dissolved HCO 3 to CO 2 by acidification and subsequent measurement of the produced CO 2. The instrument has a linear response in the range 0.025–10 μmol HCO 3 , which enables measurements in fresh urine and plasma samples down to 5 μl. The values from plasma were compared to measurements made on 65 μl whole blood in an automatic blood gas analyzer and found not to differ significantly. Compared to currently commercially available instruments applying the same principles to measure total CO 2, this study provides a simple and robust alternative which even can be used on smaller sample volumes.

Original languageEnglish
JournalPflügers Archiv - European Journal of Physiology
Volume469
Issue number10
Pages (from-to)1267-1275
ISSN0031-6768
DOIs
Publication statusPublished - 1. Oct 2017
Externally publishedYes

Keywords

  • Acid/base status
  • Bicarbonate
  • NDIR
  • Total CO
  • Urine

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