Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

Richard K P Benninger, Bruno Vanherberghen, Stephen Young, Sabrina B Taner, Fiona J Culley, Tim Schnyder, Mark A A Neil, Daniel Wüstner, Paul M W French, Daniel M Davis, Björn Onfelt

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal.
Original languageEnglish
JournalBiophysical Journal
Volume96
Issue number2
Pages (from-to)L13-5
ISSN0006-3495
DOIs
Publication statusPublished - 1. Jan 2009

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Membranes
Cell Membrane
Optical Imaging
Photons

Keywords

  • Algorithms
  • Cell Line, Transformed
  • Cell Line, Tumor
  • Cell Membrane
  • Cytoplasmic Vesicles
  • Fluorescence
  • Glycosylphosphatidylinositols
  • HLA-C Antigens
  • Humans
  • Immunological Synapses
  • Killer Cells, Natural
  • Microscopy, Confocal
  • Receptors, KIR2DL1
  • Software

Cite this

Benninger, R. K. P., Vanherberghen, B., Young, S., Taner, S. B., Culley, F. J., Schnyder, T., ... Onfelt, B. (2009). Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses. Biophysical Journal, 96(2), L13-5. https://doi.org/10.1016/j.bpj.2008.10.005
Benninger, Richard K P ; Vanherberghen, Bruno ; Young, Stephen ; Taner, Sabrina B ; Culley, Fiona J ; Schnyder, Tim ; Neil, Mark A A ; Wüstner, Daniel ; French, Paul M W ; Davis, Daniel M ; Onfelt, Björn. / Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses. In: Biophysical Journal. 2009 ; Vol. 96, No. 2. pp. L13-5.
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abstract = "We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal.",
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Benninger, RKP, Vanherberghen, B, Young, S, Taner, SB, Culley, FJ, Schnyder, T, Neil, MAA, Wüstner, D, French, PMW, Davis, DM & Onfelt, B 2009, 'Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses', Biophysical Journal, vol. 96, no. 2, pp. L13-5. https://doi.org/10.1016/j.bpj.2008.10.005

Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses. / Benninger, Richard K P; Vanherberghen, Bruno; Young, Stephen; Taner, Sabrina B; Culley, Fiona J; Schnyder, Tim; Neil, Mark A A; Wüstner, Daniel; French, Paul M W; Davis, Daniel M; Onfelt, Björn.

In: Biophysical Journal, Vol. 96, No. 2, 01.01.2009, p. L13-5.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

AU - Benninger, Richard K P

AU - Vanherberghen, Bruno

AU - Young, Stephen

AU - Taner, Sabrina B

AU - Culley, Fiona J

AU - Schnyder, Tim

AU - Neil, Mark A A

AU - Wüstner, Daniel

AU - French, Paul M W

AU - Davis, Daniel M

AU - Onfelt, Björn

PY - 2009/1/1

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N2 - We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal.

AB - We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal.

KW - Algorithms

KW - Cell Line, Transformed

KW - Cell Line, Tumor

KW - Cell Membrane

KW - Cytoplasmic Vesicles

KW - Fluorescence

KW - Glycosylphosphatidylinositols

KW - HLA-C Antigens

KW - Humans

KW - Immunological Synapses

KW - Killer Cells, Natural

KW - Microscopy, Confocal

KW - Receptors, KIR2DL1

KW - Software

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DO - 10.1016/j.bpj.2008.10.005

M3 - Journal article

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VL - 96

SP - L13-5

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

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ER -