@inbook{42a4d7f3a1c44891a584367589d79d2e,
title = "LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue",
abstract = "Liquid chromatography-mass spectrometry (LC-MS) is a widely used methodology for measuring lipids at a global level. Combined with an optimal extraction method LC-MS enables the detection and characterization of a wide range of lipid species even of low abundance. Here, we describe two extraction- and LC-MS-based quantitative analytical methods for lipid, acyl-CoA, and acyl-carnitine analyses from either mouse C2C12 myotubes or mouse skeletal tissue. We also describe the use of 13C16-palmitate and its incorporation into acyl-carnitines to show how stable isotope tracers are metabolized within cells and therefore can be implemented for lipidomic flux analysis.",
keywords = "Acyl-CoA, Acyl-carnitine, C2C12 myotubes, Labeling, Lipidomics, Liquid chromatography, Mass spectrometry, Palmitate, Skeletal muscle tissue, Stable isotope tracers",
author = "Marta Moreno-Torres and Havelund, {Jesper F} and Faergeman, {Nils J}",
year = "2019",
doi = "10.1007/978-1-4939-8897-6_12",
language = "English",
isbn = "978-1-4939-8896-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
number = "1889",
pages = "213--228",
editor = "R{\o}nning, {Sissel Beate}",
booktitle = "Myogenesis",
address = "United States",
}