Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance

Philipp M.G. Löffler; Nikolaj A. Risgaard; Bettina L. Svendsen; Katrine A. Jepsen; Alexander Rabe; Stefan Vogel

doi:10.1039/d3cc02793g

Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance

Philipp M.G. Löffler, Nikolaj A. Risgaard, Bettina L. Svendsen, Katrine A. Jepsen, Alexander Rabe, Stefan Vogel^*

^*Corresponding author for this work

University of Southern Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers in situ. The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.

Original language	English
Journal	Chemical Communications
Volume	59
Issue number	70
Pages (from-to)	10548-10551
ISSN	1359-7345
DOIs	https://doi.org/10.1039/d3cc02793g
Publication status	Published - 2023

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10.1039/d3cc02793g

1 Ph.D. thesis

Programmed Chemistry in Lipid-Nanocompartments
Risgaard, N., 9. Sept 2022, Syddansk Universitet. Det Naturvidenskabelige Fakultet. 333 p.
Research output: Thesis › Ph.D. thesis

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title = "Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance",

abstract = "Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers in situ. The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.",

author = "L{\"o}ffler, {Philipp M.G.} and Risgaard, {Nikolaj A.} and Svendsen, {Bettina L.} and Jepsen, {Katrine A.} and Alexander Rabe and Stefan Vogel",

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language = "English",

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T1 - Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance

AU - Löffler, Philipp M.G.

AU - Risgaard, Nikolaj A.

AU - Svendsen, Bettina L.

AU - Jepsen, Katrine A.

AU - Rabe, Alexander

AU - Vogel, Stefan

PY - 2023

Y1 - 2023

N2 - Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers in situ. The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.

AB - Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers in situ. The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.

U2 - 10.1039/d3cc02793g

DO - 10.1039/d3cc02793g

M3 - Journal article

C2 - 37566388

AN - SCOPUS:85168566080

SN - 1359-7345

VL - 59

SP - 10548

EP - 10551

JO - Chemical Communications

JF - Chemical Communications

IS - 70

ER -

Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance

Abstract

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Related research output

Programmed Chemistry in Lipid-Nanocompartments

Cite this