Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis

Chandrakant Ramkrishna Malwade, Maria Cinta Roda Serrat, Knud Villy Christensen, Xavier Fretté, Lars Porskjær Christensen

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Phycocyanobilin (PCB) is an important linear tetrapyrrolic molecule for food as well as pharmaceutical industry. It is obtained from blue-green algae, where it is attached covalently to phycobiliproteins (C-PC and APC) present in the light harvesting complexes. In this work, cleavage of PCB from phycobiliproteins present in the extract of Arthrospira platensis by methanolysis is investigated. Different initial concentrations (25 mg/mL, 10 mg/mL, and 5 mg/mL) of proteins are used in order to investigate the effect of protein aggregation on process yield. A kinetic model is developed by fitting the experimental data for methanolysis. Results show that the kinetics follows a pseudo first order kinetics and remains unaffected due to the different initial concentration of
phycobiliproteins. Moreover, yield of PCB in the cleavage process is found to be
proportional to the initial concentration of phycobiliproteins.
Original languageEnglish
Title of host publicationComputer - Aided Chemical Engineering
EditorsZdravko Kravanja, Miloš Bogataj
Place of PublicationAmsterdam
PublisherElsevier Masson
Publication date12. Jun 2016
ISBN (Print)978-0-444-63428-3
ISBN (Electronic)978-0-444-63873-1
Publication statusPublished - 12. Jun 2016
Event26th European Symposium on Computer Aided Process Engineering - Portoroz, Slovenia
Duration: 12. Jun 201615. Jun 2016
Conference number: 26


Conference26th European Symposium on Computer Aided Process Engineering
SeriesComputer Aided Chemical Engineering


  • Methanolysis
  • reaction kinetics
  • food color
  • phycocyanobilin
  • Arthrospira platensis


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