Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45

James A Wohlschlegel; Suman K Dhar; Tatyana A Prokhorova; Anindya Dutta; Johannes C Walter

doi:10.1016/S1097-2765(02)00456-2

Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45

James A Wohlschlegel, Suman K Dhar, Tatyana A Prokhorova, Anindya Dutta^*, Johannes C Walter

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Current models suggest that the replication initiation factor Mcm10 is required for association of Mcm2-7 with origins of replication to generate the prereplicative complex (pre-RC). Here we report that Xenopus Mcm10 (XMcm10) is not required for origin binding of XMcm2-7. Instead, the chromatin binding of XMcm10 at the onset of DNA replication requires chromatin-bound XMcm2-7, and it is independent of Cdk2 and Cdc7. In the absence of XMcm10, XCdc45 binding, XRPA binding, and initiation-dependent plasmid supercoiling are blocked. Therefore, XMcm10 performs its function after pre-RC assembly and before origin unwinding. As one of the earliest known pre-RC activation steps, chromatin binding of XMcm10 is an attractive target for regulation by cell cycle checkpoints.

Original language	English
Journal	Molecular Cell
Volume	9
Issue number	2
Pages (from-to)	233-240
ISSN	1097-2765
DOIs	https://doi.org/10.1016/S1097-2765(02)00456-2
Publication status	Published - Feb 2002
Externally published	Yes

Keywords

Animals
Carrier Proteins/metabolism
Cell Cycle
Cell Cycle Proteins/genetics
Chromatin/metabolism
DNA/metabolism
DNA Replication/physiology
DNA-Binding Proteins/metabolism
Egg Proteins/metabolism
Expressed Sequence Tags
Kinetics
Macromolecular Substances
Minichromosome Maintenance Proteins
Nuclear Proteins/metabolism
Oocytes/metabolism
Protein Binding
Protein-Serine-Threonine Kinases/physiology
Replication Origin/genetics
Saccharomyces cerevisiae Proteins
Xenopus Proteins/metabolism
Xenopus laevis/metabolism

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title = "Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45",

abstract = "Current models suggest that the replication initiation factor Mcm10 is required for association of Mcm2-7 with origins of replication to generate the prereplicative complex (pre-RC). Here we report that Xenopus Mcm10 (XMcm10) is not required for origin binding of XMcm2-7. Instead, the chromatin binding of XMcm10 at the onset of DNA replication requires chromatin-bound XMcm2-7, and it is independent of Cdk2 and Cdc7. In the absence of XMcm10, XCdc45 binding, XRPA binding, and initiation-dependent plasmid supercoiling are blocked. Therefore, XMcm10 performs its function after pre-RC assembly and before origin unwinding. As one of the earliest known pre-RC activation steps, chromatin binding of XMcm10 is an attractive target for regulation by cell cycle checkpoints.",

keywords = "Animals, Carrier Proteins/metabolism, Cell Cycle, Cell Cycle Proteins/genetics, Chromatin/metabolism, DNA/metabolism, DNA Replication/physiology, DNA-Binding Proteins/metabolism, Egg Proteins/metabolism, Expressed Sequence Tags, Kinetics, Macromolecular Substances, Minichromosome Maintenance Proteins, Nuclear Proteins/metabolism, Oocytes/metabolism, Protein Binding, Protein-Serine-Threonine Kinases/physiology, Replication Origin/genetics, Saccharomyces cerevisiae Proteins, Xenopus Proteins/metabolism, Xenopus laevis/metabolism",

author = "Wohlschlegel, {James A} and Dhar, {Suman K} and Prokhorova, {Tatyana A} and Anindya Dutta and Walter, {Johannes C}",

year = "2002",

month = feb,

doi = "10.1016/S1097-2765(02)00456-2",

language = "English",

volume = "9",

pages = "233--240",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "2",

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TY - JOUR

T1 - Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45

AU - Wohlschlegel, James A

AU - Dhar, Suman K

AU - Prokhorova, Tatyana A

AU - Dutta, Anindya

AU - Walter, Johannes C

PY - 2002/2

Y1 - 2002/2

N2 - Current models suggest that the replication initiation factor Mcm10 is required for association of Mcm2-7 with origins of replication to generate the prereplicative complex (pre-RC). Here we report that Xenopus Mcm10 (XMcm10) is not required for origin binding of XMcm2-7. Instead, the chromatin binding of XMcm10 at the onset of DNA replication requires chromatin-bound XMcm2-7, and it is independent of Cdk2 and Cdc7. In the absence of XMcm10, XCdc45 binding, XRPA binding, and initiation-dependent plasmid supercoiling are blocked. Therefore, XMcm10 performs its function after pre-RC assembly and before origin unwinding. As one of the earliest known pre-RC activation steps, chromatin binding of XMcm10 is an attractive target for regulation by cell cycle checkpoints.

AB - Current models suggest that the replication initiation factor Mcm10 is required for association of Mcm2-7 with origins of replication to generate the prereplicative complex (pre-RC). Here we report that Xenopus Mcm10 (XMcm10) is not required for origin binding of XMcm2-7. Instead, the chromatin binding of XMcm10 at the onset of DNA replication requires chromatin-bound XMcm2-7, and it is independent of Cdk2 and Cdc7. In the absence of XMcm10, XCdc45 binding, XRPA binding, and initiation-dependent plasmid supercoiling are blocked. Therefore, XMcm10 performs its function after pre-RC assembly and before origin unwinding. As one of the earliest known pre-RC activation steps, chromatin binding of XMcm10 is an attractive target for regulation by cell cycle checkpoints.

KW - Animals

KW - Carrier Proteins/metabolism

KW - Cell Cycle

KW - Cell Cycle Proteins/genetics

KW - Chromatin/metabolism

KW - DNA/metabolism

KW - DNA Replication/physiology

KW - DNA-Binding Proteins/metabolism

KW - Egg Proteins/metabolism

KW - Expressed Sequence Tags

KW - Kinetics

KW - Macromolecular Substances

KW - Minichromosome Maintenance Proteins

KW - Nuclear Proteins/metabolism

KW - Oocytes/metabolism

KW - Protein Binding

KW - Protein-Serine-Threonine Kinases/physiology

KW - Replication Origin/genetics

KW - Saccharomyces cerevisiae Proteins

KW - Xenopus Proteins/metabolism

KW - Xenopus laevis/metabolism

U2 - 10.1016/S1097-2765(02)00456-2

DO - 10.1016/S1097-2765(02)00456-2

M3 - Journal article

C2 - 11864598

SN - 1097-2765

VL - 9

SP - 233

EP - 240

JO - Molecular Cell

JF - Molecular Cell

IS - 2

ER -

Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45

Abstract

Keywords

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