Isolation of Kupffer Cells and Hepatocytes from a Single Mouse Liver

Marcela Aparicio-Vergara, Michaela Tencerova, Cecilia Morgantini, Emelie Barreby, Myriam Aouadi

Research output: Chapter in Book/Report/Conference proceedingBook chapterCommunication

Abstract

Liver perfusion is a common technique used to isolate parenchymal and non-parenchymal liver cells for in vitro experiments. This method allows hepatic cells to be separated based on their size and weight, by centrifugation using a density gradient. To date, other methods allow the isolation of only one viable hepatic cellular fraction from a single mouse; either parenchymal (hepatocytes) or non-parenchymal cells (i.e., Kupffer cells or hepatic stellate cells). Here, we describe a method to isolate both hepatocytes and Kupffer cells from a single mouse liver, thereby providing the unique advantage of studying different liver cell types that have been isolated from the same organism.

Original languageEnglish
Title of host publicationAlpha-1 Antitrypsin Deficiency : Methods and Protocols
EditorsFlorie Borel, Christian Mueller
Volume1639
PublisherHumana Press
Publication date2017
Pages161-171
ISBN (Print)978149397169
ISBN (Electronic)9781493971633
DOIs
Publication statusPublished - 2017
SeriesMethods in Molecular Biology
Volume1639
ISSN1064-3745

Keywords

  • Journal Article

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