Isoforms of retinol binding protein 4 (RBP4) are increased in chronic diseases of the kidney but not of the liver

Simone K Frey, Britta Nagl, Andrea Henze, Jens Raila, Beate Schlosser, Thomas Berg, Martin Tepel, Walter Zidek, Martin O Weickert, Andreas F H Pfeiffer, Florian J Schweigert

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The levels of retinol-binding protein 4 (RBP4) - the carrier protein for Vitamin A in plasma - are tightly regulated under healthy circumstances. The kidney, the main site of RBP4 catabolism, contributes to an elevation of RBP4 levels during chronic kidney disease (CKD) whereas during chronic liver disease (CLD) RBP4 levels decrease. Little is known about RBP4 isoforms including apo-RBP4, holo-RBP4 as well as RBP4 truncated at the C-terminus (RBP4-L and RBP4-LL) except that RBP4 isoforms have been reported to be increased in hemodialysis patients. Since it is not known whether CLD influence RBP4 isoforms, we investigated RBP4 levels, apo- and holo-RBP4 as well as RBP4-L and RBP4-LL in plasma of 36 patients suffering from CKD, in 55 CLD patients and in 50 control subjects. RBP4 was determined by ELISA and apo- and holo-RBP4 by native polyacrylamide gel electrophoresis (PAGE). RBP4-L and RBP4-LL were analyzed after immunoprecipitation by mass spectrometry (MALDI-TOF-MS).
Original languageEnglish
JournalLipids in Health and Disease
Volume7
Pages (from-to)29
ISSN1476-511X
DOIs
Publication statusPublished - 1. Jan 2008

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Retinol-Binding Proteins
Liver
Protein Isoforms
Liver Diseases
Plasmas

Keywords

  • Anthropometry
  • Apoproteins
  • Case-Control Studies
  • Chronic Disease
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Kidney Diseases
  • Kidney Function Tests
  • Liver Diseases
  • Liver Function Tests
  • Male
  • Middle Aged
  • Mutant Proteins
  • Prealbumin
  • Protein Isoforms
  • Retinol-Binding Proteins, Plasma
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Vitamin A

Cite this

Frey, Simone K ; Nagl, Britta ; Henze, Andrea ; Raila, Jens ; Schlosser, Beate ; Berg, Thomas ; Tepel, Martin ; Zidek, Walter ; Weickert, Martin O ; Pfeiffer, Andreas F H ; Schweigert, Florian J. / Isoforms of retinol binding protein 4 (RBP4) are increased in chronic diseases of the kidney but not of the liver. In: Lipids in Health and Disease. 2008 ; Vol. 7. pp. 29.
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abstract = "The levels of retinol-binding protein 4 (RBP4) - the carrier protein for Vitamin A in plasma - are tightly regulated under healthy circumstances. The kidney, the main site of RBP4 catabolism, contributes to an elevation of RBP4 levels during chronic kidney disease (CKD) whereas during chronic liver disease (CLD) RBP4 levels decrease. Little is known about RBP4 isoforms including apo-RBP4, holo-RBP4 as well as RBP4 truncated at the C-terminus (RBP4-L and RBP4-LL) except that RBP4 isoforms have been reported to be increased in hemodialysis patients. Since it is not known whether CLD influence RBP4 isoforms, we investigated RBP4 levels, apo- and holo-RBP4 as well as RBP4-L and RBP4-LL in plasma of 36 patients suffering from CKD, in 55 CLD patients and in 50 control subjects. RBP4 was determined by ELISA and apo- and holo-RBP4 by native polyacrylamide gel electrophoresis (PAGE). RBP4-L and RBP4-LL were analyzed after immunoprecipitation by mass spectrometry (MALDI-TOF-MS).",
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author = "Frey, {Simone K} and Britta Nagl and Andrea Henze and Jens Raila and Beate Schlosser and Thomas Berg and Martin Tepel and Walter Zidek and Weickert, {Martin O} and Pfeiffer, {Andreas F H} and Schweigert, {Florian J}",
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Frey, SK, Nagl, B, Henze, A, Raila, J, Schlosser, B, Berg, T, Tepel, M, Zidek, W, Weickert, MO, Pfeiffer, AFH & Schweigert, FJ 2008, 'Isoforms of retinol binding protein 4 (RBP4) are increased in chronic diseases of the kidney but not of the liver', Lipids in Health and Disease, vol. 7, pp. 29. https://doi.org/10.1186/1476-511X-7-29

Isoforms of retinol binding protein 4 (RBP4) are increased in chronic diseases of the kidney but not of the liver. / Frey, Simone K; Nagl, Britta; Henze, Andrea; Raila, Jens; Schlosser, Beate; Berg, Thomas; Tepel, Martin; Zidek, Walter; Weickert, Martin O; Pfeiffer, Andreas F H; Schweigert, Florian J.

In: Lipids in Health and Disease, Vol. 7, 01.01.2008, p. 29.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Isoforms of retinol binding protein 4 (RBP4) are increased in chronic diseases of the kidney but not of the liver

AU - Frey, Simone K

AU - Nagl, Britta

AU - Henze, Andrea

AU - Raila, Jens

AU - Schlosser, Beate

AU - Berg, Thomas

AU - Tepel, Martin

AU - Zidek, Walter

AU - Weickert, Martin O

AU - Pfeiffer, Andreas F H

AU - Schweigert, Florian J

PY - 2008/1/1

Y1 - 2008/1/1

N2 - The levels of retinol-binding protein 4 (RBP4) - the carrier protein for Vitamin A in plasma - are tightly regulated under healthy circumstances. The kidney, the main site of RBP4 catabolism, contributes to an elevation of RBP4 levels during chronic kidney disease (CKD) whereas during chronic liver disease (CLD) RBP4 levels decrease. Little is known about RBP4 isoforms including apo-RBP4, holo-RBP4 as well as RBP4 truncated at the C-terminus (RBP4-L and RBP4-LL) except that RBP4 isoforms have been reported to be increased in hemodialysis patients. Since it is not known whether CLD influence RBP4 isoforms, we investigated RBP4 levels, apo- and holo-RBP4 as well as RBP4-L and RBP4-LL in plasma of 36 patients suffering from CKD, in 55 CLD patients and in 50 control subjects. RBP4 was determined by ELISA and apo- and holo-RBP4 by native polyacrylamide gel electrophoresis (PAGE). RBP4-L and RBP4-LL were analyzed after immunoprecipitation by mass spectrometry (MALDI-TOF-MS).

AB - The levels of retinol-binding protein 4 (RBP4) - the carrier protein for Vitamin A in plasma - are tightly regulated under healthy circumstances. The kidney, the main site of RBP4 catabolism, contributes to an elevation of RBP4 levels during chronic kidney disease (CKD) whereas during chronic liver disease (CLD) RBP4 levels decrease. Little is known about RBP4 isoforms including apo-RBP4, holo-RBP4 as well as RBP4 truncated at the C-terminus (RBP4-L and RBP4-LL) except that RBP4 isoforms have been reported to be increased in hemodialysis patients. Since it is not known whether CLD influence RBP4 isoforms, we investigated RBP4 levels, apo- and holo-RBP4 as well as RBP4-L and RBP4-LL in plasma of 36 patients suffering from CKD, in 55 CLD patients and in 50 control subjects. RBP4 was determined by ELISA and apo- and holo-RBP4 by native polyacrylamide gel electrophoresis (PAGE). RBP4-L and RBP4-LL were analyzed after immunoprecipitation by mass spectrometry (MALDI-TOF-MS).

KW - Anthropometry

KW - Apoproteins

KW - Case-Control Studies

KW - Chronic Disease

KW - Electrophoresis, Polyacrylamide Gel

KW - Female

KW - Humans

KW - Kidney Diseases

KW - Kidney Function Tests

KW - Liver Diseases

KW - Liver Function Tests

KW - Male

KW - Middle Aged

KW - Mutant Proteins

KW - Prealbumin

KW - Protein Isoforms

KW - Retinol-Binding Proteins, Plasma

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Vitamin A

U2 - 10.1186/1476-511X-7-29

DO - 10.1186/1476-511X-7-29

M3 - Journal article

VL - 7

SP - 29

JO - Lipids in Health and Disease

JF - Lipids in Health and Disease

SN - 1476-511X

ER -