Investigation of transfollicular caffeine penetration using microdialysis on ex vivo porcine ear skin

Anna Lena Klein*, Markus Lubda, Per Stahl Skov, Annika Vogt, Cornelia M. Keck, Jürgen Lademann, Ingeborg Beckers, Jörg von Hagen, Alexa Patzelt

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The aim of this study was to develop an ex vivo method that allows to quantify the transfollicular penetration of topically applied substances by combining microdialysis and selective follicular closure with varnish. An experimental setup with three skin areas on ex vivo intact porcine ear skin was designed (varnish on hair follicle, varnish next to hair follicle, no varnish). On each area, 10 µl/cm2 caffeine-hydroxyethyl-cellulose-gel was applied. Samples were collected for 22 h by microdialysis. After sampling, the skin layers were separated, homogenized and caffeine was quantified by high pressure liquid chromatography (HPLC) in all samples. Potential impact of the varnish placed next to the follicle by tension on the follicle during the drying process was monitored by a microscopic setup and could be excluded. The microdialysis and homogenization study showed a significantly reduced penetration of caffeine when the hair follicles were closed. In areas with open hair follicles caffeine was detected already in the first ten minutes after application. The reported novel combination of two methods is suitable to investigate ex vivo transfollicular penetration. Possible impact of the closure material in the control area can be ruled out by adjusting the design of the control area in future studies.

Original languageEnglish
JournalEuropean journal of pharmaceutics and biopharmaceutics
Volume157
Pages (from-to)1-8
ISSN0939-6411
DOIs
Publication statusPublished - Dec 2020

Keywords

  • Caffeine
  • Follicular closing
  • Hair follicle
  • High pressure liquid chromatography (HPLC)
  • Microdialysis
  • Skin penetration

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