Investigating temporal changes in the yeast phosphoproteome upon fatty acid starvation

Dennis Pultz, Martin Bennetzen, Jens S. Andersen, Nils J. Færgeman

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Investigating stemporal changes in the yeast phosphoproteome upon fatty acid starvation

Dennis Pultz*, Martin Bennetzen*, Jens S. Andersen and Nils J.Færgeman. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark, 5230

Reducing food intake to induce under nutrition but not malnutrition extends the life span of multiple species, ranging from single-celled organisms like yeast to mammals. This increase in longevity by dietary restriction (DR) is coupled to profound beneficial effects on age-related pathology. Despite the number of studies on DR and the physiological changes DR induces, only little is known about the genetics and signalling networks which regulate the DR response.
We have recently shown that inhibition of fatty acid synthesis in Saccharomyces cerevisiae results in a dependency on autophagy in maintaining normal life span. We further believe that the nutrient sensor TOR1 is central in the regulation and orchestration of the downstream cellular response upon fatty acid starvation.
By use of mass spectrometry and a SILAC (stable isotope labelling by amino acids in cell culture) based approach we wish to unravel the temporal changes in the phosphoproteome coupling fatty acid starvation to autophagy and starvation related cellular responses in general. By use of this approach we have identified approximately 2000 phosphorylation sites of which more than 400 have been identified as being regulated.
Here, we present results showing the temporal behaviour of downstream regulated processes as well as functional analysis indicating the dependency on bioinformatically predicted key regulators of the starvation response.
Translated title of the contributionInvestigating stemporal changes in the yeast phosphoproteome upon fatty acid starvation
Original languageEnglish
Publication date2011
Publication statusPublished - 2011
  • Keystone Symposia: Autophagy

    Dennis Pultz (Speaker)

    27. Mar 20111. Apr 2011

    Activity: Talks and presentationsTalks and presentations in private or public companies

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