Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis

Research output: Chapter in Book/Report/Conference proceedingBook chapterCommunication

Abstract

The aim of this chapter is to give a short introduction to peptide analysis by mass spectrometry (MS) and interpretation of fragment mass spectra. Through examples and guidelines we demonstrate how to understand and validate search results and how to perform de novo sequencing based on the often very complex fragmentation pattern obtained by tandem mass spectrometry (also referred to as MSMS). The focus is on simple rules for interpretation of MSMS spectra of tryptic as well as non-tryptic peptides.

Original languageEnglish
Title of host publicationPeptide antibodies : methods and protocols
Volume1348
PublisherHumana Press
Publication date2015
Pages83-102
ISBN (Print)978-1-4939-2998-6
DOIs
Publication statusPublished - 2015
SeriesMethods in Molecular Biology
Volume1348
ISSN1064-3745

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Tandem Mass Spectrometry
Peptides
Guidelines

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Hjernø, K., & Højrup, P. (2015). Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis. In Peptide antibodies: methods and protocols (Vol. 1348, pp. 83-102). Humana Press. Methods in Molecular Biology, Vol.. 1348 https://doi.org/10.1007/978-1-4939-2999-3_10
Hjernø, Karin ; Højrup, Peter. / Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis. Peptide antibodies: methods and protocols. Vol. 1348 Humana Press, 2015. pp. 83-102 (Methods in Molecular Biology, Vol. 1348).
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Hjernø, K & Højrup, P 2015, Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis. in Peptide antibodies: methods and protocols. vol. 1348, Humana Press, Methods in Molecular Biology, vol. 1348, pp. 83-102. https://doi.org/10.1007/978-1-4939-2999-3_10

Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis. / Hjernø, Karin; Højrup, Peter.

Peptide antibodies: methods and protocols. Vol. 1348 Humana Press, 2015. p. 83-102 (Methods in Molecular Biology, Vol. 1348).

Research output: Chapter in Book/Report/Conference proceedingBook chapterCommunication

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Hjernø K, Højrup P. Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis. In Peptide antibodies: methods and protocols. Vol. 1348. Humana Press. 2015. p. 83-102. (Methods in Molecular Biology, Vol. 1348). https://doi.org/10.1007/978-1-4939-2999-3_10