Interpretation of Tandem Mass Spectrometry (MS-MS) Spectra for Peptide Analysis

Karin Hjernø, Peter Højrup*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Abstract

The aim of this chapter is to give a short introduction to peptide analysis by mass spectrometry (MS) and interpretation of fragment mass spectra. Through examples and guidelines, we will demonstrate how to understand and validate search results and how to perform de novo sequencing based on the often very complex fragmentation pattern obtained by tandem mass spectrometry (also referred to as MSMS). The focus will be on simple rules for interpretation of MSMS spectra of tryptic as well as non-tryptic peptides.

Original languageEnglish
Title of host publicationPeptide Antibodies
EditorsG Houen
Volume2821
PublisherHumana Press
Publication date2024
Pages91-110
ISBN (Print)978-1-0716-3913-9
ISBN (Electronic)978-1-0716-3914-6
DOIs
Publication statusPublished - 2024
SeriesMethods in Molecular Biology
Volume2821
ISSN1064-3745

Keywords

  • De novo sequencing
  • Fragmentation
  • Mass spectrometry
  • MS
  • Tandem mass spectra
  • Validation

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