Interplay of membrane crosslinking and curvature induction by annexins

Anna Mularski, Stine Lauritzen Sønder, Anne Sofie Busk Heitmann, Mayank Prakash Pandey, Himanshu Khandelia, Jesper Nylandsted, Adam Cohen Simonsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Efficient plasma membrane repair (PMR) is required to repair damage sustained in the cellular life cycle. The annexin family of proteins, involved in PMR, are activated by Ca2+ influx from extracellular media at the site of injury. Mechanistic studies of the annexins have been overwhelmingly performed using a single annexin, despite the recruitment of multiple annexins to membrane damage sites in living cells. Hence, we investigate the effect of the presence of the crosslinking annexins, annexin A1, A2 and A6 (ANXA1, ANXA2 and ANXA6) on the membrane curvature induction of annexin A4 (ANXA4) in model membrane systems. Our data support a mechanistic model of PMR where ANXA4 induced membrane curvature and ANXA6 crosslinking promotes wound closure. The model now can be expanded to include ANXA1 and ANXA2 as specialist free edge membrane crosslinkers that act in concert with ANXA4 induced curvature and ANXA6 crosslinking.

Original languageEnglish
Article number22568
JournalScientific Reports
Volume12
ISSN2045-2322
DOIs
Publication statusPublished - Dec 2022

Keywords

  • Annexin A1/metabolism
  • Annexin A4/metabolism
  • Annexins/metabolism
  • Cell Membrane/metabolism
  • Models, Biological
  • Wound Healing

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