Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material

F. Schiele*, J. Muller, E. Colinet, G. Siest, P. Arzoglou, H. Brettschneider, D. H. Calam, F. Ceriotti, G. Ferard, J. Frei, A. Hadjivassiliou, J. C.M. Hafkenscheid, M. Horder, F. J. Gella, R. Rej, E. Schmidt, A. Vassault, V. Viette

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We present the results of a study on performance of a reference material for alanine aminotransferase (ALT, EC 2.6.1.2) and the corresponding IFCC- approved method in an interlaboratory trial involving 13 laboratories. The ALT material was partly purified from pig heart (specific activity, 150 kU/g) and was essentially free of six potentially contaminating enzyme activities, including aspartate aminotransferase (EC 2.6.1.1). The partly purified ALT was lyophilized in a triethanolamine-buffered matrix, pH 6.4, containing bovine serum albumin and saccharose. Under these conditions, the predicted yearly loss of activity was 0.02% at 4 °C and <0.01% at -20 °C. The final blank-corrected results of the accepted set of data gave a mean (SD) of 128.5 (5.1) U/L. The among-laboratory SD was 4.6 U/L and the within-laboratory SD was 2.0 U/L. The certified ALT catalytic concentration in the reconstituted material was 129 U/L with a 0.95 confidence interval of ± 4 U/L.

Original languageEnglish
JournalClinical Chemistry
Volume38
Issue number12
Pages (from-to)2365-2371
Number of pages7
ISSN0009-9147
Publication statusPublished - 1. Dec 1992

Fingerprint Dive into the research topics of 'Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material'. Together they form a unique fingerprint.

Cite this