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Inhibition of yeast growth during long term exposure to laser light around 1064 nm

  • Thomas Aabo
  • , Ivan R. Perch-Nielsen
  • , Jeppe Seidelin Dam
  • , Darwin Palima
  • , Henrik Siegumfeldt
  • , Jesper Glückstad
  • , Nils Arneborg
  • Technical University of Denmark

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.
Original languageEnglish
Title of host publicationComplex Light and Optical Forces III
PublisherSPIE - International Society for Optical Engineering
Publication date2009
Article number722706
DOIs
Publication statusPublished - 2009
Externally publishedYes
EventSPIE OPTO: INTEGRATED OPTOELECTRONIC DEVICES - San Jose, United States
Duration: 24. Jan 200929. Jan 2009

Conference

ConferenceSPIE OPTO: INTEGRATED OPTOELECTRONIC DEVICES
Country/TerritoryUnited States
CitySan Jose
Period24/01/200929/01/2009
SeriesProceedings of SPIE, the International Society for Optical Engineering
Volume7227
ISSN0277-786X

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