Increased detectability of somatic changes in the DNA from human tumours after probing with "synthetic" and "genome-derived" hypervariable multilocus probes

P J Lagoda; G Seitz; J T Epplen; O G Issinger

doi:10.1007/BF00210667

Increased detectability of somatic changes in the DNA from human tumours after probing with "synthetic" and "genome-derived" hypervariable multilocus probes

P J Lagoda
, G Seitz
, J T Epplen
, O G Issinger

Universitätsklinikum des Saarlandes

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

DNA fingerprinting with two minisatellite (33.15, M13) and two simple repeat probes [(GACA)4, (CAC)5/(GTG)s] was performed to screen for somatic changes in the DNA from various solid human tumours in comparison with constitutional DNA from the same patient. Loss of bands or changes in band intensities were observed. Together the probes 33.15 and (CAC)5/(GTG)5 detected deviating fingerprint patterns in 63% of the colorectal carcinomas investigated. In mammary and stomach carcinomas, only 1/11 and 2/11 tumours, respectively, showed differences with either of the three probes, 33.15, (GACA)4 and (CAC)5/(GTG)5.

Original language	English
Journal	Human Genetics
Volume	84
Issue number	1
Pages (from-to)	35-40
ISSN	0340-6717
DOIs	https://doi.org/10.1007/BF00210667
Publication status	Published - 1. Dec 1989
Externally published	Yes

Keywords

Breast Neoplasms
Colonic Neoplasms
DNA
DNA Probes
DNA, Neoplasm
Female
Fibrocystic Breast Disease
Genetic Variation
Humans
Neoplasms
Nucleotide Mapping
Rectal Neoplasms
Restriction Mapping
Stomach Neoplasms

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title = "Increased detectability of somatic changes in the DNA from human tumours after probing with {"}synthetic{"} and {"}genome-derived{"} hypervariable multilocus probes",

abstract = "DNA fingerprinting with two minisatellite (33.15, M13) and two simple repeat probes [(GACA)4, (CAC)5/(GTG)s] was performed to screen for somatic changes in the DNA from various solid human tumours in comparison with constitutional DNA from the same patient. Loss of bands or changes in band intensities were observed. Together the probes 33.15 and (CAC)5/(GTG)5 detected deviating fingerprint patterns in 63\% of the colorectal carcinomas investigated. In mammary and stomach carcinomas, only 1/11 and 2/11 tumours, respectively, showed differences with either of the three probes, 33.15, (GACA)4 and (CAC)5/(GTG)5.",

keywords = "Breast Neoplasms, Colonic Neoplasms, DNA, DNA Probes, DNA, Neoplasm, Female, Fibrocystic Breast Disease, Genetic Variation, Humans, Neoplasms, Nucleotide Mapping, Rectal Neoplasms, Restriction Mapping, Stomach Neoplasms",

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year = "1989",

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T1 - Increased detectability of somatic changes in the DNA from human tumours after probing with "synthetic" and "genome-derived" hypervariable multilocus probes

AU - Lagoda, P J

AU - Seitz, G

AU - Epplen, J T

AU - Issinger, O G

PY - 1989/12/1

Y1 - 1989/12/1

N2 - DNA fingerprinting with two minisatellite (33.15, M13) and two simple repeat probes [(GACA)4, (CAC)5/(GTG)s] was performed to screen for somatic changes in the DNA from various solid human tumours in comparison with constitutional DNA from the same patient. Loss of bands or changes in band intensities were observed. Together the probes 33.15 and (CAC)5/(GTG)5 detected deviating fingerprint patterns in 63% of the colorectal carcinomas investigated. In mammary and stomach carcinomas, only 1/11 and 2/11 tumours, respectively, showed differences with either of the three probes, 33.15, (GACA)4 and (CAC)5/(GTG)5.

AB - DNA fingerprinting with two minisatellite (33.15, M13) and two simple repeat probes [(GACA)4, (CAC)5/(GTG)s] was performed to screen for somatic changes in the DNA from various solid human tumours in comparison with constitutional DNA from the same patient. Loss of bands or changes in band intensities were observed. Together the probes 33.15 and (CAC)5/(GTG)5 detected deviating fingerprint patterns in 63% of the colorectal carcinomas investigated. In mammary and stomach carcinomas, only 1/11 and 2/11 tumours, respectively, showed differences with either of the three probes, 33.15, (GACA)4 and (CAC)5/(GTG)5.

KW - Breast Neoplasms

KW - Colonic Neoplasms

KW - DNA

KW - DNA Probes

KW - DNA, Neoplasm

KW - Female

KW - Fibrocystic Breast Disease

KW - Genetic Variation

KW - Humans

KW - Neoplasms

KW - Nucleotide Mapping

KW - Rectal Neoplasms

KW - Restriction Mapping

KW - Stomach Neoplasms

U2 - 10.1007/BF00210667

DO - 10.1007/BF00210667

M3 - Journal article

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SN - 0340-6717

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SP - 35

EP - 40

JO - Human Genetics

JF - Human Genetics

IS - 1

ER -

Increased detectability of somatic changes in the DNA from human tumours after probing with "synthetic" and "genome-derived" hypervariable multilocus probes

Abstract

Keywords

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