Increased activation of Iba1+ microglia in pediatric epilepsy patients with Rasmussen's encephalitis compared with cortical dysplasia and tuberous sclerosis complex

Martin Wirenfeldt; Ryan Clare; Spencer Tung; Alexander Bottini; Gary W Mathern; Harry V Vinters

doi:10.1016/j.nbd.2009.02.015

Increased activation of Iba1+ microglia in pediatric epilepsy patients with Rasmussen's encephalitis compared with cortical dysplasia and tuberous sclerosis complex

Martin Wirenfeldt, Ryan Clare, Spencer Tung, Alexander Bottini, Gary W Mathern, Harry V Vinters

University of California, Los Angeles

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Microgliosis is prominent in Rasmussen's encephalitis (RE), a disease with severe seizure activity. However, it is unclear if microglial activation is similar with different histopathologic substrates. Iba1-immunolabelled microglial activation was assessed in neocortex from pediatric epilepsy surgery patients with RE (n=8), cortical dysplasia (CD; n=6) and tuberous sclerosis complex (TSC; n=6). Microglial reactivity was increased, in severely affected RE areas (29% labeling) compared with minimally affected areas of RE cases (15%) and cases of TSC (14%) and CD (12%). There was no qualitative association of Iba1 immunolabelling with the presence of CD8(+) cytotoxic T-cells and no statistical association with clinical epilepsy variables, such as seizure duration or frequency. Iba1 appears to be an excellent marker for detecting extensive microglial activation in patients with RE. In addition, this study supports the notion that Iba1-labeled microglial activation is increased in patients with active RE, compared with cases of CD and TSC.

Original language	English
Journal	Neurobiology of Disease
Volume	34
Issue number	3
Pages (from-to)	432-40
Number of pages	9
ISSN	0969-9961
DOIs	https://doi.org/10.1016/j.nbd.2009.02.015
Publication status	Published - Jun 2009
Externally published	Yes

Keywords

Adolescent
Brain/pathology
CD8-Positive T-Lymphocytes/physiology
Calcium-Binding Proteins
Cell Proliferation
Child
Child, Preschool
Cohort Studies
DNA-Binding Proteins/metabolism
Encephalitis/complications
Epilepsy/complications
Female
Humans
Infant
Infant, Newborn
Male
Malformations of Cortical Development/complications
Microfilament Proteins
Microglia/pathology
Seizures/complications
Severity of Illness Index
Tuberous Sclerosis/complications

Documents & Links

10.1016/j.nbd.2009.02.015

Cite this

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title = "Increased activation of Iba1+ microglia in pediatric epilepsy patients with Rasmussen's encephalitis compared with cortical dysplasia and tuberous sclerosis complex",

abstract = "Microgliosis is prominent in Rasmussen's encephalitis (RE), a disease with severe seizure activity. However, it is unclear if microglial activation is similar with different histopathologic substrates. Iba1-immunolabelled microglial activation was assessed in neocortex from pediatric epilepsy surgery patients with RE (n=8), cortical dysplasia (CD; n=6) and tuberous sclerosis complex (TSC; n=6). Microglial reactivity was increased, in severely affected RE areas (29% labeling) compared with minimally affected areas of RE cases (15%) and cases of TSC (14%) and CD (12%). There was no qualitative association of Iba1 immunolabelling with the presence of CD8(+) cytotoxic T-cells and no statistical association with clinical epilepsy variables, such as seizure duration or frequency. Iba1 appears to be an excellent marker for detecting extensive microglial activation in patients with RE. In addition, this study supports the notion that Iba1-labeled microglial activation is increased in patients with active RE, compared with cases of CD and TSC.",

keywords = "Adolescent, Brain/pathology, CD8-Positive T-Lymphocytes/physiology, Calcium-Binding Proteins, Cell Proliferation, Child, Child, Preschool, Cohort Studies, DNA-Binding Proteins/metabolism, Encephalitis/complications, Epilepsy/complications, Female, Humans, Infant, Infant, Newborn, Male, Malformations of Cortical Development/complications, Microfilament Proteins, Microglia/pathology, Seizures/complications, Severity of Illness Index, Tuberous Sclerosis/complications",

author = "Martin Wirenfeldt and Ryan Clare and Spencer Tung and Alexander Bottini and Mathern, {Gary W} and Vinters, {Harry V}",

year = "2009",

month = jun,

doi = "10.1016/j.nbd.2009.02.015",

language = "English",

volume = "34",

pages = "432--40",

journal = "Neurobiology of Disease",

issn = "0969-9961",

publisher = "Academic Press",

number = "3",

}

Increased activation of Iba1+ microglia in pediatric epilepsy patients with Rasmussen's encephalitis compared with cortical dysplasia and tuberous sclerosis complex. / Wirenfeldt, Martin; Clare, Ryan; Tung, Spencer et al.
In: Neurobiology of Disease, Vol. 34, No. 3, 06.2009, p. 432-40.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Increased activation of Iba1+ microglia in pediatric epilepsy patients with Rasmussen's encephalitis compared with cortical dysplasia and tuberous sclerosis complex

AU - Wirenfeldt, Martin

AU - Clare, Ryan

AU - Tung, Spencer

AU - Bottini, Alexander

AU - Mathern, Gary W

AU - Vinters, Harry V

PY - 2009/6

Y1 - 2009/6

N2 - Microgliosis is prominent in Rasmussen's encephalitis (RE), a disease with severe seizure activity. However, it is unclear if microglial activation is similar with different histopathologic substrates. Iba1-immunolabelled microglial activation was assessed in neocortex from pediatric epilepsy surgery patients with RE (n=8), cortical dysplasia (CD; n=6) and tuberous sclerosis complex (TSC; n=6). Microglial reactivity was increased, in severely affected RE areas (29% labeling) compared with minimally affected areas of RE cases (15%) and cases of TSC (14%) and CD (12%). There was no qualitative association of Iba1 immunolabelling with the presence of CD8(+) cytotoxic T-cells and no statistical association with clinical epilepsy variables, such as seizure duration or frequency. Iba1 appears to be an excellent marker for detecting extensive microglial activation in patients with RE. In addition, this study supports the notion that Iba1-labeled microglial activation is increased in patients with active RE, compared with cases of CD and TSC.

AB - Microgliosis is prominent in Rasmussen's encephalitis (RE), a disease with severe seizure activity. However, it is unclear if microglial activation is similar with different histopathologic substrates. Iba1-immunolabelled microglial activation was assessed in neocortex from pediatric epilepsy surgery patients with RE (n=8), cortical dysplasia (CD; n=6) and tuberous sclerosis complex (TSC; n=6). Microglial reactivity was increased, in severely affected RE areas (29% labeling) compared with minimally affected areas of RE cases (15%) and cases of TSC (14%) and CD (12%). There was no qualitative association of Iba1 immunolabelling with the presence of CD8(+) cytotoxic T-cells and no statistical association with clinical epilepsy variables, such as seizure duration or frequency. Iba1 appears to be an excellent marker for detecting extensive microglial activation in patients with RE. In addition, this study supports the notion that Iba1-labeled microglial activation is increased in patients with active RE, compared with cases of CD and TSC.

KW - Adolescent

KW - Brain/pathology

KW - CD8-Positive T-Lymphocytes/physiology

KW - Calcium-Binding Proteins

KW - Cell Proliferation

KW - Child

KW - Child, Preschool

KW - Cohort Studies

KW - DNA-Binding Proteins/metabolism

KW - Encephalitis/complications

KW - Epilepsy/complications

KW - Female

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Male

KW - Malformations of Cortical Development/complications

KW - Microfilament Proteins

KW - Microglia/pathology

KW - Seizures/complications

KW - Severity of Illness Index

KW - Tuberous Sclerosis/complications

U2 - 10.1016/j.nbd.2009.02.015

DO - 10.1016/j.nbd.2009.02.015

M3 - Journal article

C2 - 19285133

SN - 0969-9961

VL - 34

SP - 432

EP - 440

JO - Neurobiology of Disease

JF - Neurobiology of Disease

IS - 3

ER -

Increased activation of Iba1+ microglia in pediatric epilepsy patients with Rasmussen's encephalitis compared with cortical dysplasia and tuberous sclerosis complex

Abstract

Keywords

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Cite this