In-vitro chondrogenic potential of synovial stem cells and chondrocytes allocated for autologous chondrocyte implantation - a comparison: Synovial stem cells as an alternative cell source for autologous chondrocyte implantation

Eva Johanna Kubosch*, Emanuel Heidt, Philipp Niemeyer, Anke Bernstein, Norbert P Südkamp, Hagen Schmal

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Purpose: The use of passaged chondrocytes is the current standard for autologous chondrocyte implantation (ACI). De-differentiation due to amplification and donor site morbidity are known drawbacks highlighting the need for alternative cell sources. Methods: Via clinically validated flow cytometry analysis, we compared the expression of human stem cell and cartilage markers (collagen type 2 (Col2), aggrecan (ACAN), CD44) of chondrocytes (CHDR), passaged chondrocytes for ACI (CellGenix™), bone marrow derived mesenchymal stem cells (BMSC), and synovial derived stem cells (SDSC). Results: Primary, human BMSC and SDSC revealed similar adipogenic, osteogenic, and chondrogenic differentiation potential and stem cell marker expression. However, the expression of the chondrogenic markers Col2 and ACAN was statistically significant higher in SDSC. CHDR and SDSC expressed ACAN and CD44 equally, but Col2 was expressed more strongly on the SDSC surface. The marker expression of SDSC from osteoarthritic joints (Kellgren-Lawrence score ≥3) versus normal knees (Kellgren-Lawrence score ≤2) did not differ. Similarly, there was no difference between temporarily frozen and fresh SDSC. Col2 and ACAN surface expression declined with further passaging, whereas CD44 remained unchanged. We observed the same effect after reducing the serum content. When comparing CHDR for ACI with SDSC of the same passage (P2/3), both Col2 and ACAN, correlating with clinical outcome, were expressed higher in SDSC. Conclusions: In summary, SDSC demonstrated high differentiation potential and a stable chondrogenic phenotype. They might therefore be better suitable for ACI than BMSC or passaged CHDR.

Original languageEnglish
JournalInternational Orthopaedics
Volume41
Issue number5
Pages (from-to)991–998
ISSN0341-2695
DOIs
Publication statusPublished - May 2017

Keywords

  • Autologous chondrocyte implantation
  • Cartilage repair
  • Cell quality
  • Collagen type II
  • Synoviocytes/metabolism
  • Humans
  • Middle Aged
  • Male
  • Collagen Type II/metabolism
  • Mesenchymal Stem Cells/cytology
  • Flow Cytometry
  • Orthopedic Procedures
  • Cell Differentiation/physiology
  • Aggrecans/metabolism
  • Aged, 80 and over
  • Adult
  • Female
  • Chondrogenesis
  • Transplantation, Autologous/methods
  • Cells, Cultured
  • Hyaluronan Receptors/metabolism
  • Osteoarthritis, Knee/metabolism
  • Chondrocytes/metabolism
  • Stem Cells/metabolism
  • Cartilage/metabolism
  • Aged
  • Osteogenesis

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