Immunohistochemical Localization of Deleted in Malignant Brain Tumors 1 in Normal Human Tissues

Anders Bathum Nexøe, Andreas Arnholdt Pedersen, Sebastian von Huth, Sönke Detlefsen, Pernille Lund Hansen, Uffe Holmskov*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Deleted in malignant brain tumors 1 (DMBT1) is part of the innate immune system and is expressed on mucosal surfaces in various tissues throughout the human body. However, to date, the localization of DMBT1 has not been investigated systematically and comprehensively in normal human tissues. In this study, we analyzed the mRNA expression of DMBT1 in human tissue by quantitative real-time PCR and examined its localization and distribution in the tissue by immunohistochemical staining using the monoclonal DMBT1 antibody HYB213-6. Anti-ovalbumin was used as an isotype control. The highest level of mRNA expression of DMBT1 was found in the small intestine, and the expression level was high throughout the luminal digestive tract. The expression of DMBT1 was especially high in the luminal digestive tract and salivary glands. The lowest expression level was found in the spleen. Immunohistochemical staining showed a high expression level of DMBT1 on mucosal surfaces throughout the body. There was a clear correlation between the mRNA expression and immunohistochemical expression of DMBT1 in the tissue. DMBT1 is strongly expressed on mucosal surfaces and in salivary glands
Original languageEnglish
JournalJournal of Histochemistry and Cytochemistry
Volume68
Issue number6
Pages (from-to)377-387
ISSN0022-1554
DOIs
Publication statusPublished - 1. Jun 2020

Keywords

  • GP340
  • innate immunity
  • mucosal immunity
  • pattern recognition
  • scavenger receptor cysteine-rich domain

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