Imaging and modeling of acute pressure-induced changes of collagen and elastin microarchitectures in pig and human resistance arteries

Maria Bloksgaard, Thomas M Leurgans, Bart Spronck, Maarten Hg Heusinkveld, Bjarne Thorsted, Kristoffer Rosenstand, Inger Nissen, Ulla Melchior Hansen, Jonathan R Brewer, Luis A Bagatolli, Lars M Rasmussen, Akhmadjon Irmukhamedov, Koen D Reesink, Jo G R De Mey

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Abstract

The impact of disease-related changes in the extracellular matrix (ECM) on the mechanical properties of human resistance arteries largely remains to be established. Resistance arteries from both pig and human parietal pericardium (PRA) display a different ECM microarchitecture compared with frequently used rodent mesenteric arteries. We hypothesized that the biaxial mechanics of PRA mirror pressure-induced changes in the ECM microarchitecture. This was tested using isolated pig PRA as a model system, integrating vital imaging, pressure myography, and mathematical modeling. Collagenase and elastase digestions were applied to evaluate the load-bearing roles of collagen and elastin, respectively. The incremental elastic modulus linearly related to the straightness of adventitial collagen fibers circumferentially and longitudinally (both R 2 ≥ 0.99), whereas there was a nonlinear relationship to the internal elastic lamina elastin fiber branching angles. Mathematical modeling suggested a collagen recruitment strain (means ± SE) of 1.1 ± 0.2 circumferentially and 0.20 ± 0.01 longitudinally, corresponding to a pressure of ~40 mmHg, a finding supported by the vital imaging. The integrated method was tested on human PRA to confirm its validity. These showed limited circumferential distensibility and elongation and a collagen recruitment strain of 0.8 ± 0.1 circumferentially and 0.06 ± 0.02 longitudinally, reached at a distending pressure below 20 mmHg. This was confirmed by vital imaging showing negligible microarchitectural changes of elastin and collagen upon pressurization. In conclusion, we show here, for the first time in resistance arteries, a quantitative relationship between pressure-induced changes in the extracellular matrix and the arterial wall mechanics. The strength of the integrated methods invites for future detailed studies of microvascular pathologies. NEW & NOTEWORTHY This is the first study to quantitatively relate pressure-induced microstructural changes in resistance arteries to the mechanics of their wall. Principal findings using a pig model system were confirmed in human arteries. The combined methods provide a strong tool for future hypothesis-driven studies of microvascular pathologies.

Original languageEnglish
JournalAmerican Journal of Physiology: Heart and Circulatory Physiology
Volume313
Issue number1
Pages (from-to)H164–H178
ISSN0363-6135
DOIs
Publication statusPublished - 1. Jul 2017

Keywords

  • Journal Article
  • Twophoton excitation fluorescence microscopy
  • Extracellular matrix
  • Incremental elastic (Young’s) modulus
  • Collagen recruitment
  • Blood Pressure/physiology
  • Arterioles/diagnostic imaging
  • Models, Cardiovascular
  • Mechanotransduction, Cellular/physiology
  • Elastic Modulus/physiology
  • Stress, Mechanical
  • Collagen/physiology
  • Animals
  • Vascular Resistance/physiology
  • Swine
  • Computer Simulation
  • Extracellular Matrix/physiology
  • Elastin/physiology

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