Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays

Martin W. Nielsen, Paoli Marcatili, Thomas Sicheritz-Ponten, Tim K. Jensen, Claus Schafer-Nielsen, Mette Boye, Morten Nielsen, Kirstine Klitgaard

    Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

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    Abstract

    Bovine digital dermatitis (DD) is the most important infectious cause of lameness in dairy cattle, and a major contributing factor to welfare problems and economic losses in the dairy cattle industry worldwide. DD is a disease that involves chronic dermal inflammatory processes and destruction of collagenous and connective tissues. Multiple Treponema species, many of which are not-yet-cultivable, are strongly implicated in disease progression. Despite the economic and welfare importance of this disease, no effective vaccine is available; and there is presently very little knowledge concerning efficacious immunoprophylactic antigens against DD.
    It is highly likely that DD-associated treponemes possess considerable antigenic variation, as cows exhibit a variable humoral response against different isolates of Treponema. Hence, combinations of antigens from multiple Treponema species should be used for the development of disease prevention measures. As treponemes from DD lesions are extremely difficult to culture, identification of these antigens is challenging. To circumvent this problem, we studied the in situ gene expression patterns of the microbiome in DD-affected skin lesions and the host antibody response directed at the site of infection. By metatranscriptomics we measured the in situ genome-wide transcriptome of the bacterial population in DD-affected skin lesions from 21 dairy cows. From the transcriptome data, we identified a panel of Treponema genes that were highly expressed in multiple animals, and we monitored the host immune response to these target genes using high-density peptide microarrays. By this approach, we identified a small group of antigenic proteins, which were expressed in the majority of the samples, and demonstrated antigenicity when screened against sera from infected animal. Future studies will show if these proteins represent candidates for the development of novel biomarkers or vaccines

    Conference

    Conference2nd International Symposium on Alternatives to Antibiotics, Challenges and Solutions in Animal Production
    CountryFrance
    CityParis
    Period12/12/201615/12/2016
    Internet address

    Cite this

    Nielsen, M. W., Marcatili, P., Sicheritz-Ponten, T., Jensen, T. K., Schafer-Nielsen, C., Boye, M., ... Klitgaard, K. (2017). Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays. Abstract from 2nd International Symposium on Alternatives to Antibiotics, Challenges and Solutions in Animal Production, Paris, France.
    Nielsen, Martin W. ; Marcatili, Paoli ; Sicheritz-Ponten, Thomas ; Jensen, Tim K. ; Schafer-Nielsen, Claus ; Boye, Mette ; Nielsen, Morten ; Klitgaard, Kirstine . / Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays. Abstract from 2nd International Symposium on Alternatives to Antibiotics, Challenges and Solutions in Animal Production, Paris, France.1 p.
    @conference{f2428c0ba6c349388628eb5382060e58,
    title = "Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays",
    abstract = "Bovine digital dermatitis (DD) is the most important infectious cause of lameness in dairy cattle, and a major contributing factor to welfare problems and economic losses in the dairy cattle industry worldwide. DD is a disease that involves chronic dermal inflammatory processes and destruction of collagenous and connective tissues. Multiple Treponema species, many of which are not-yet-cultivable, are strongly implicated in disease progression. Despite the economic and welfare importance of this disease, no effective vaccine is available; and there is presently very little knowledge concerning efficacious immunoprophylactic antigens against DD.It is highly likely that DD-associated treponemes possess considerable antigenic variation, as cows exhibit a variable humoral response against different isolates of Treponema. Hence, combinations of antigens from multiple Treponema species should be used for the development of disease prevention measures. As treponemes from DD lesions are extremely difficult to culture, identification of these antigens is challenging. To circumvent this problem, we studied the in situ gene expression patterns of the microbiome in DD-affected skin lesions and the host antibody response directed at the site of infection. By metatranscriptomics we measured the in situ genome-wide transcriptome of the bacterial population in DD-affected skin lesions from 21 dairy cows. From the transcriptome data, we identified a panel of Treponema genes that were highly expressed in multiple animals, and we monitored the host immune response to these target genes using high-density peptide microarrays. By this approach, we identified a small group of antigenic proteins, which were expressed in the majority of the samples, and demonstrated antigenicity when screened against sera from infected animal. Future studies will show if these proteins represent candidates for the development of novel biomarkers or vaccines",
    author = "Nielsen, {Martin W.} and Paoli Marcatili and Thomas Sicheritz-Ponten and Jensen, {Tim K.} and Claus Schafer-Nielsen and Mette Boye and Morten Nielsen and Kirstine Klitgaard",
    year = "2017",
    month = "12",
    day = "12",
    language = "English",
    note = "null ; Conference date: 12-12-2016 Through 15-12-2016",
    url = "https://www.onehealthcommission.org/index.cfm/37526/79719/2nd_international_symposium_on_alternatives_to_antibiotics_challenges_and_solutions_in_animal_production",

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    Nielsen, MW, Marcatili, P, Sicheritz-Ponten, T, Jensen, TK, Schafer-Nielsen, C, Boye, M, Nielsen, M & Klitgaard, K 2017, 'Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays', Paris, France, 12/12/2016 - 15/12/2016, .

    Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays. / Nielsen, Martin W.; Marcatili, Paoli; Sicheritz-Ponten, Thomas; Jensen, Tim K.; Schafer-Nielsen, Claus; Boye, Mette; Nielsen, Morten; Klitgaard, Kirstine .

    2017. Abstract from 2nd International Symposium on Alternatives to Antibiotics, Challenges and Solutions in Animal Production, Paris, France.

    Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

    TY - ABST

    T1 - Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays

    AU - Nielsen, Martin W.

    AU - Marcatili, Paoli

    AU - Sicheritz-Ponten, Thomas

    AU - Jensen, Tim K.

    AU - Schafer-Nielsen, Claus

    AU - Boye, Mette

    AU - Nielsen, Morten

    AU - Klitgaard, Kirstine

    PY - 2017/12/12

    Y1 - 2017/12/12

    N2 - Bovine digital dermatitis (DD) is the most important infectious cause of lameness in dairy cattle, and a major contributing factor to welfare problems and economic losses in the dairy cattle industry worldwide. DD is a disease that involves chronic dermal inflammatory processes and destruction of collagenous and connective tissues. Multiple Treponema species, many of which are not-yet-cultivable, are strongly implicated in disease progression. Despite the economic and welfare importance of this disease, no effective vaccine is available; and there is presently very little knowledge concerning efficacious immunoprophylactic antigens against DD.It is highly likely that DD-associated treponemes possess considerable antigenic variation, as cows exhibit a variable humoral response against different isolates of Treponema. Hence, combinations of antigens from multiple Treponema species should be used for the development of disease prevention measures. As treponemes from DD lesions are extremely difficult to culture, identification of these antigens is challenging. To circumvent this problem, we studied the in situ gene expression patterns of the microbiome in DD-affected skin lesions and the host antibody response directed at the site of infection. By metatranscriptomics we measured the in situ genome-wide transcriptome of the bacterial population in DD-affected skin lesions from 21 dairy cows. From the transcriptome data, we identified a panel of Treponema genes that were highly expressed in multiple animals, and we monitored the host immune response to these target genes using high-density peptide microarrays. By this approach, we identified a small group of antigenic proteins, which were expressed in the majority of the samples, and demonstrated antigenicity when screened against sera from infected animal. Future studies will show if these proteins represent candidates for the development of novel biomarkers or vaccines

    AB - Bovine digital dermatitis (DD) is the most important infectious cause of lameness in dairy cattle, and a major contributing factor to welfare problems and economic losses in the dairy cattle industry worldwide. DD is a disease that involves chronic dermal inflammatory processes and destruction of collagenous and connective tissues. Multiple Treponema species, many of which are not-yet-cultivable, are strongly implicated in disease progression. Despite the economic and welfare importance of this disease, no effective vaccine is available; and there is presently very little knowledge concerning efficacious immunoprophylactic antigens against DD.It is highly likely that DD-associated treponemes possess considerable antigenic variation, as cows exhibit a variable humoral response against different isolates of Treponema. Hence, combinations of antigens from multiple Treponema species should be used for the development of disease prevention measures. As treponemes from DD lesions are extremely difficult to culture, identification of these antigens is challenging. To circumvent this problem, we studied the in situ gene expression patterns of the microbiome in DD-affected skin lesions and the host antibody response directed at the site of infection. By metatranscriptomics we measured the in situ genome-wide transcriptome of the bacterial population in DD-affected skin lesions from 21 dairy cows. From the transcriptome data, we identified a panel of Treponema genes that were highly expressed in multiple animals, and we monitored the host immune response to these target genes using high-density peptide microarrays. By this approach, we identified a small group of antigenic proteins, which were expressed in the majority of the samples, and demonstrated antigenicity when screened against sera from infected animal. Future studies will show if these proteins represent candidates for the development of novel biomarkers or vaccines

    M3 - Conference abstract for conference

    ER -

    Nielsen MW, Marcatili P, Sicheritz-Ponten T, Jensen TK, Schafer-Nielsen C, Boye M et al. Identification of Common Bacterial Antigenic Markers From Bovine Digital Dermatitis Lesions Using Meta-Transcriptomics in Combination With High-Density Peptide-Microarrays. 2017. Abstract from 2nd International Symposium on Alternatives to Antibiotics, Challenges and Solutions in Animal Production, Paris, France.