Identification of Abnormal Stem Cells Using Raman Spectroscopy

Linda Harkness, Sergey M Novikov, Jonas Beermann, Sergey I Bozhevolnyi, Moustapha Kassem

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label-free method for rapid and sensitive detection of changes in cells' bio-molecular composition. Here, we report that by using Raman spectroscopy, we were able to map the distribution of different biomolecules within 2 types of stem cells: adult human bone marrow-derived stromal stem cells and human embryonic stem cells and to identify reproducible differences in Raman's spectral characteristics that distinguished genetically abnormal and transformed stem cells from their normal counterparts. Raman microscopy can be prospectively employed as a method for identifying abnormal stem cells in ex vivo cultures prior to clinical transplantation.
Original languageEnglish
JournalStem Cells and Development
Volume21
Issue number12
Pages (from-to)2152-9
Number of pages9
ISSN1547-3287
DOIs
Publication statusPublished - 2012

Fingerprint

Microscopy
Stromal Cells
Human Embryonic Stem Cells

Cite this

@article{3adf0cd270494b96a7f6109018390997,
title = "Identification of Abnormal Stem Cells Using Raman Spectroscopy",
abstract = "The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label-free method for rapid and sensitive detection of changes in cells' bio-molecular composition. Here, we report that by using Raman spectroscopy, we were able to map the distribution of different biomolecules within 2 types of stem cells: adult human bone marrow-derived stromal stem cells and human embryonic stem cells and to identify reproducible differences in Raman's spectral characteristics that distinguished genetically abnormal and transformed stem cells from their normal counterparts. Raman microscopy can be prospectively employed as a method for identifying abnormal stem cells in ex vivo cultures prior to clinical transplantation.",
author = "Linda Harkness and Novikov, {Sergey M} and Jonas Beermann and Bozhevolnyi, {Sergey I} and Moustapha Kassem",
year = "2012",
doi = "10.1089/scd.2011.0600",
language = "English",
volume = "21",
pages = "2152--9",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Incorporated",
number = "12",

}

Identification of Abnormal Stem Cells Using Raman Spectroscopy. / Harkness, Linda; Novikov, Sergey M; Beermann, Jonas; Bozhevolnyi, Sergey I; Kassem, Moustapha.

In: Stem Cells and Development, Vol. 21, No. 12, 2012, p. 2152-9.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Identification of Abnormal Stem Cells Using Raman Spectroscopy

AU - Harkness, Linda

AU - Novikov, Sergey M

AU - Beermann, Jonas

AU - Bozhevolnyi, Sergey I

AU - Kassem, Moustapha

PY - 2012

Y1 - 2012

N2 - The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label-free method for rapid and sensitive detection of changes in cells' bio-molecular composition. Here, we report that by using Raman spectroscopy, we were able to map the distribution of different biomolecules within 2 types of stem cells: adult human bone marrow-derived stromal stem cells and human embryonic stem cells and to identify reproducible differences in Raman's spectral characteristics that distinguished genetically abnormal and transformed stem cells from their normal counterparts. Raman microscopy can be prospectively employed as a method for identifying abnormal stem cells in ex vivo cultures prior to clinical transplantation.

AB - The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label-free method for rapid and sensitive detection of changes in cells' bio-molecular composition. Here, we report that by using Raman spectroscopy, we were able to map the distribution of different biomolecules within 2 types of stem cells: adult human bone marrow-derived stromal stem cells and human embryonic stem cells and to identify reproducible differences in Raman's spectral characteristics that distinguished genetically abnormal and transformed stem cells from their normal counterparts. Raman microscopy can be prospectively employed as a method for identifying abnormal stem cells in ex vivo cultures prior to clinical transplantation.

U2 - 10.1089/scd.2011.0600

DO - 10.1089/scd.2011.0600

M3 - Journal article

VL - 21

SP - 2152

EP - 2159

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 12

ER -