Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Toke Peter Krogager, Lone Vendel Nielsen, Steffen Bak, Clifford Young, Carla Ferreri, Ole Nørregaard Jensen, Peter Højrup, Vladimiros Thoma, Ida Thøgersen, Jan J. Enghild

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value
Original languageEnglish
JournalJournal of Proteomics
Volume75
Issue number9
Pages (from-to)2685-96
Number of pages12
ISSN1874-3919
DOIs
Publication statusPublished - 2012

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