Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Toke Peter Krogager, Lone Vendel Nielsen, Steffen Bak, Clifford Young, Carla Ferreri, Ole Nørregaard Jensen, Peter Højrup, Vladimiros Thoma, Ida Thøgersen, Jan J. Enghild

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value
Original languageEnglish
JournalJournal of Proteomics
Volume75
Issue number9
Pages (from-to)2685-96
Number of pages12
ISSN1874-3919
DOIs
Publication statusPublished - 2012

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Isotope Labeling
Dietary Fats
Biomarkers
Cell culture
Isotopes
Labeling
Fats
Trans Fatty Acids
Amino Acids
Serum-Free Culture Media
Oleic Acid
Microarrays
Electrophoresis
Gene expression
Liver
Lipoproteins
Blood Proteins
Gels
Cells
Plasmas

Cite this

Krogager, Toke Peter ; Nielsen, Lone Vendel ; Bak, Steffen ; Young, Clifford ; Ferreri, Carla ; Jensen, Ole Nørregaard ; Højrup, Peter ; Thoma, Vladimiros ; Thøgersen, Ida ; Enghild, Jan J. / Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1). In: Journal of Proteomics. 2012 ; Vol. 75, No. 9. pp. 2685-96.
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abstract = "Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value",
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Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1). / Krogager, Toke Peter; Nielsen, Lone Vendel; Bak, Steffen; Young, Clifford; Ferreri, Carla; Jensen, Ole Nørregaard; Højrup, Peter; Thoma, Vladimiros; Thøgersen, Ida; Enghild, Jan J.

In: Journal of Proteomics, Vol. 75, No. 9, 2012, p. 2685-96.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

AU - Krogager, Toke Peter

AU - Nielsen, Lone Vendel

AU - Bak, Steffen

AU - Young, Clifford

AU - Ferreri, Carla

AU - Jensen, Ole Nørregaard

AU - Højrup, Peter

AU - Thoma, Vladimiros

AU - Thøgersen, Ida

AU - Enghild, Jan J.

N1 - Copyright © 2012 Elsevier B.V. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value

AB - Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value

U2 - 10.1016/j.jprot.2012.03.023

DO - 10.1016/j.jprot.2012.03.023

M3 - Journal article

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VL - 75

SP - 2685

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JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 9

ER -