TY - JOUR
T1 - Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)
AU - Krogager, Toke Peter
AU - Nielsen, Lone Vendel
AU - Bak, Steffen
AU - Young, Clifford
AU - Ferreri, Carla
AU - Jensen, Ole Nørregaard
AU - Højrup, Peter
AU - Thoma, Vladimiros
AU - Thøgersen, Ida
AU - Enghild, Jan J.
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2012
Y1 - 2012
N2 - Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value
AB - Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value
U2 - 10.1016/j.jprot.2012.03.023
DO - 10.1016/j.jprot.2012.03.023
M3 - Journal article
C2 - 22483997
SN - 1874-3919
VL - 75
SP - 2685
EP - 2696
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 9
ER -