High–performance liquid chromatography of clomipramine and metabolites in human plasma and urine

Karin Kramer Nielsen*, Kim Brøsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


An isocratic, high-performance liquid chromatography method has been developed for simultaneous determination of the tricyclic antidepressant clomipramine and six metabolites: desmethylclomipramine, 2- and 8-hydroxy-clomipramine, 2- and 8-hydroxydesmethylclomipramine and didesmethyl-clomipramine in plasma and urine. In addition, clomipramine N-oxide was determined in the unconjugated fraction of urine. The method is based on a three-step liquid-liquid extraction, the chromatographic eluent was 30% acetonitrile and 70% aqueous sodium perchlorate solution, pH 2.5, and the UV detection was performed at 220 nm. The method was applied to the analysis of samples followed by oral doses of clomipramine to patients and healthy volunteers.

Original languageEnglish
JournalTherapeutic Drug Monitoring
Issue number2
Pages (from-to)122-128
Number of pages7
Publication statusPublished - 1. Jan 1993



  • Clomipramine
  • High-performance liquid chromatography
  • Human plasma and urine
  • Seven metabolites
  • Ultraviolet detection

Cite this