Highly regular axon array in a microfluidic chip

Ayako Yamada, Maéva Vignes, Cécile Bureau, Bastien Venzac, Stéphanie Descroix, Jean Louis Viovy, Catherine Villard*, Jean Michel Peyrin, Laurent Malaquin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

We aim at finding the most efficient way to guide axons. We thus compare three different techniques: i) In micro-contact printing, poly-lysine micro-patterns are printed on a flat substrate, providing a chemical constraint; ii) In micro-grooves, microstructures are homogeneously coated with poly-lysine, yielding a physical constraint; iii) In in-mold-patterning, finally microstructures with poly-lysine only at the bottom surface of the structures are prepared, yielding a hybrid constraint. Neurons were grown on guiding tracks of these substrates, which were integrated in microfluidic chips. The in-mold patterning technique provides the best axon guidance, yielding highly regular and fully accessible on-chip axon arrays.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Publication date2015
Pages555-557
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Externally publishedYes
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 25. Oct 201529. Oct 2015

Conference

Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country/TerritoryKorea, Republic of
CityGyeongju
Period25/10/201529/10/2015
SponsorACS Publications, Analytical Chemistry, American Elements, Aquatech Co., Ltd and Takasago Fluidic Systems, ASK, CapitalBio Technology

Keywords

  • Axon guidance
  • In-mold patterning
  • Mouse cortical neuron

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