High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry

Marcus Ståhlman, Christer S. Ejsing, Kirill Tarasov, Jeanna Perman, Jan Borén, Kim Ekroos

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Technological advances in mass spectrometry and meticulous method development have produced several shotgun lipidomic approaches capable of characterizing lipid species by direct analysis of total lipid extracts. Shotgun lipidomics by hybrid quadrupole time-of-flight mass spectrometry allows the absolute quantification of hundreds of molecular glycerophospholipid species, glycerolipid species, sphingolipid species and sterol lipids. Future applications in clinical cohort studies demand detailed lipid molecule information and the application of high-throughput lipidomics platforms. In this review we describe a novel high-throughput shotgun lipidomic platform based on 96-well robot-assisted lipid extraction, automated sample infusion by mircofluidic-based nanoelectrospray ionization, and quantitative multiple precursor ion scanning analysis on a quadrupole time-of-flight mass spectrometer. Using this platform to compile comprehensive lipid arrays associated with metabolic dysfunctions is a powerful strategy for pinpointing the mechanistic details by which alterations in tissue-specific lipid metabolism are directly linked to the etiology of many lipid-mediated disorders.
Original languageEnglish
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number26
Pages (from-to)2664-72
Number of pages9
ISSN1570-0232
DOIs
Publication statusPublished - 2009

Fingerprint

Mass spectrometry
Throughput
Lipids
Glycerophospholipids
Sphingolipids
Mass spectrometers
Sterols
Ionization
Robots
Ions
Tissue
Scanning
Molecules

Keywords

  • Animals
  • Computational Biology
  • Humans
  • Lipid Metabolism, Inborn Errors
  • Lipids
  • Spectrometry, Mass, Electrospray Ionization

Cite this

Ståhlman, Marcus ; Ejsing, Christer S. ; Tarasov, Kirill ; Perman, Jeanna ; Borén, Jan ; Ekroos, Kim. / High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009 ; Vol. 877, No. 26. pp. 2664-72.
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High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry. / Ståhlman, Marcus; Ejsing, Christer S.; Tarasov, Kirill; Perman, Jeanna; Borén, Jan; Ekroos, Kim.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 26, 2009, p. 2664-72.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry

AU - Ståhlman, Marcus

AU - Ejsing, Christer S.

AU - Tarasov, Kirill

AU - Perman, Jeanna

AU - Borén, Jan

AU - Ekroos, Kim

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Y1 - 2009

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AB - Technological advances in mass spectrometry and meticulous method development have produced several shotgun lipidomic approaches capable of characterizing lipid species by direct analysis of total lipid extracts. Shotgun lipidomics by hybrid quadrupole time-of-flight mass spectrometry allows the absolute quantification of hundreds of molecular glycerophospholipid species, glycerolipid species, sphingolipid species and sterol lipids. Future applications in clinical cohort studies demand detailed lipid molecule information and the application of high-throughput lipidomics platforms. In this review we describe a novel high-throughput shotgun lipidomic platform based on 96-well robot-assisted lipid extraction, automated sample infusion by mircofluidic-based nanoelectrospray ionization, and quantitative multiple precursor ion scanning analysis on a quadrupole time-of-flight mass spectrometer. Using this platform to compile comprehensive lipid arrays associated with metabolic dysfunctions is a powerful strategy for pinpointing the mechanistic details by which alterations in tissue-specific lipid metabolism are directly linked to the etiology of many lipid-mediated disorders.

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KW - Computational Biology

KW - Humans

KW - Lipid Metabolism, Inborn Errors

KW - Lipids

KW - Spectrometry, Mass, Electrospray Ionization

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M3 - Journal article

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