Heterogeneous distribution of prokaryotes and viruses at the microscale in a tidal sediment

Cátia Carreira, Morten Larsen, Ronnie N. Glud, Corina P. D. Brussard, Mathias Middelboe

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In this study we show for the first time the microscale (mm) 2- and 3-dimensional spatial distribution and abundance of prokaryotes, viruses, and oxygen in a tidal sediment. Prokaryotes and viruses were highly heterogeneously distributed with patches of elevated abundances surrounded by areas of ca. 3-fold lower abundance within distances of <2 mm. Abundances of prokaryotes and viruses ranged from 1.3 × 109 to 4.2 × 109 cells cm−3 and 4.1 × 109 to 13.1 × 109 viruses cm−3, respectively. The results showed oxygen concentration and uptake rates to be heterogeneously distributed at the same spatial scale, with the oxygen penetration depth varying from 1.5 to 5.8 mm and with an average (±SD) diffusive oxygen uptake of 18.9 ± 6.4 mmol m−2 d−1. Locally, prokaryotes, viruses, and oxygen were found to be positively, negatively, or not correlated, but overall no significant relationship was detected. The lack of consistent spatial correlation between viruses and prokaryotes was explained by a temporal experiment using organic carbon-enriched homogenized sediment samples. Enhancement in metabolic activity and the proliferation of prokaryotes and viruses were not completely
phased. These re sults suggest that local ourishment
is likely to be an important driver of a high small-scale heterogeneity in abundance and dynamics of benthic viruses and prokaryotes. This is expected to influence the rates and regulation of benthic virus−host inter - actions and thus microbial biogeochemical cycling.
Original languageEnglish
JournalAquatic Microbial Ecology
Volume69
Pages (from-to)183-192
ISSN0948-3055
DOIs
Publication statusPublished - 2013

Cite this

@article{1b706d42f6eb467d9c25b6f63b350f2b,
title = "Heterogeneous distribution of prokaryotes and viruses at the microscale in a tidal sediment",
abstract = "In this study we show for the first time the microscale (mm) 2- and 3-dimensional spatial distribution and abundance of prokaryotes, viruses, and oxygen in a tidal sediment. Prokaryotes and viruses were highly heterogeneously distributed with patches of elevated abundances surrounded by areas of ca. 3-fold lower abundance within distances of <2 mm. Abundances of prokaryotes and viruses ranged from 1.3 × 109 to 4.2 × 109 cells cm−3 and 4.1 × 109 to 13.1 × 109 viruses cm−3, respectively. The results showed oxygen concentration and uptake rates to be heterogeneously distributed at the same spatial scale, with the oxygen penetration depth varying from 1.5 to 5.8 mm and with an average (±SD) diffusive oxygen uptake of 18.9 ± 6.4 mmol m−2 d−1. Locally, prokaryotes, viruses, and oxygen were found to be positively, negatively, or not correlated, but overall no significant relationship was detected. The lack of consistent spatial correlation between viruses and prokaryotes was explained by a temporal experiment using organic carbon-enriched homogenized sediment samples. Enhancement in metabolic activity and the proliferation of prokaryotes and viruses were not completelyphased. These re sults suggest that local ourishmentis likely to be an important driver of a high small-scale heterogeneity in abundance and dynamics of benthic viruses and prokaryotes. This is expected to influence the rates and regulation of benthic virus−host inter - actions and thus microbial biogeochemical cycling.",
author = "C{\'a}tia Carreira and Morten Larsen and Glud, {Ronnie N.} and Brussard, {Corina P. D.} and Mathias Middelboe",
year = "2013",
doi = "10.3354/ame01639",
language = "English",
volume = "69",
pages = "183--192",
journal = "Aquatic Microbial Ecology",
issn = "0948-3055",
publisher = "Inter Research",

}

Heterogeneous distribution of prokaryotes and viruses at the microscale in a tidal sediment. / Carreira, Cátia; Larsen, Morten; Glud, Ronnie N.; Brussard, Corina P. D.; Middelboe, Mathias.

In: Aquatic Microbial Ecology, Vol. 69, 2013, p. 183-192.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Heterogeneous distribution of prokaryotes and viruses at the microscale in a tidal sediment

AU - Carreira, Cátia

AU - Larsen, Morten

AU - Glud, Ronnie N.

AU - Brussard, Corina P. D.

AU - Middelboe, Mathias

PY - 2013

Y1 - 2013

N2 - In this study we show for the first time the microscale (mm) 2- and 3-dimensional spatial distribution and abundance of prokaryotes, viruses, and oxygen in a tidal sediment. Prokaryotes and viruses were highly heterogeneously distributed with patches of elevated abundances surrounded by areas of ca. 3-fold lower abundance within distances of <2 mm. Abundances of prokaryotes and viruses ranged from 1.3 × 109 to 4.2 × 109 cells cm−3 and 4.1 × 109 to 13.1 × 109 viruses cm−3, respectively. The results showed oxygen concentration and uptake rates to be heterogeneously distributed at the same spatial scale, with the oxygen penetration depth varying from 1.5 to 5.8 mm and with an average (±SD) diffusive oxygen uptake of 18.9 ± 6.4 mmol m−2 d−1. Locally, prokaryotes, viruses, and oxygen were found to be positively, negatively, or not correlated, but overall no significant relationship was detected. The lack of consistent spatial correlation between viruses and prokaryotes was explained by a temporal experiment using organic carbon-enriched homogenized sediment samples. Enhancement in metabolic activity and the proliferation of prokaryotes and viruses were not completelyphased. These re sults suggest that local ourishmentis likely to be an important driver of a high small-scale heterogeneity in abundance and dynamics of benthic viruses and prokaryotes. This is expected to influence the rates and regulation of benthic virus−host inter - actions and thus microbial biogeochemical cycling.

AB - In this study we show for the first time the microscale (mm) 2- and 3-dimensional spatial distribution and abundance of prokaryotes, viruses, and oxygen in a tidal sediment. Prokaryotes and viruses were highly heterogeneously distributed with patches of elevated abundances surrounded by areas of ca. 3-fold lower abundance within distances of <2 mm. Abundances of prokaryotes and viruses ranged from 1.3 × 109 to 4.2 × 109 cells cm−3 and 4.1 × 109 to 13.1 × 109 viruses cm−3, respectively. The results showed oxygen concentration and uptake rates to be heterogeneously distributed at the same spatial scale, with the oxygen penetration depth varying from 1.5 to 5.8 mm and with an average (±SD) diffusive oxygen uptake of 18.9 ± 6.4 mmol m−2 d−1. Locally, prokaryotes, viruses, and oxygen were found to be positively, negatively, or not correlated, but overall no significant relationship was detected. The lack of consistent spatial correlation between viruses and prokaryotes was explained by a temporal experiment using organic carbon-enriched homogenized sediment samples. Enhancement in metabolic activity and the proliferation of prokaryotes and viruses were not completelyphased. These re sults suggest that local ourishmentis likely to be an important driver of a high small-scale heterogeneity in abundance and dynamics of benthic viruses and prokaryotes. This is expected to influence the rates and regulation of benthic virus−host inter - actions and thus microbial biogeochemical cycling.

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SN - 0948-3055

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