HepG2/C3A 3D spheroids exhibit stable physiological functionality for at least 24 days after recovering from trypsinisation

Krzysztof Wrzesinski, Maria Chiara Magnone, Line Visby Hansen, Marianne Ehrhorn Kruse, Tobias Bergauer, Maria Bobadilla, Marcel Gubler, Jacques Mizrahi, Kelan Zhang, Christina Møller Andreasen, Kira Joensen, Signe Marie Andersen, Jacob Bastholm Olesen, Ove B. Schaffalitzky de Muckadell, Stephen John Fey

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Abstract

Primary human hepatocytes are widely used as an in vitro system for the assessment of drug metabolism and toxicity. Nevertheless a cell system with higher stability of physiological functions is required for the investigation of drugs’ mode of action, pathway analyses and biomarkers evaluations. We recently discovered that the human hepatocellular carcinoma cell line, HepG2/C3A, cultured as spheroids in a 3D system can recover their main functions after trypsinisation within about 18 days. The objective of this study was to investigate whether the spheroids’ metabolic functions remained stable after this recovery period. Therefore we evaluated physiological capabilities of the spheroids (cell survival, growth rate, glycogenesis, ATP, cholesterol and urea synthesis and drug metabolism) and the expression of key genes related to
the main liver pathways in spheroids cultured for an additional 24 days after full recovery (day 18). Here we show that after the recovery period, the 3D spheroid culture can provide a metabolically competent homeostatic cell model which is in equilibrium with its culture environment for more than 3 weeks. Such
a stable system could be used for the assessment of the drugs’ mode of action, for biomarkers evaluation and for any systems biology studies which require medium- to long-term stability of metabolic functions.
Original languageEnglish
JournalToxicology Research
Volume2
Issue number3
Pages (from-to)163-172
Number of pages10
ISSN2045-452X
DOIs
Publication statusPublished - May 2013

Keywords

  • Spheroids
  • 3D culture
  • toxicology
  • Physiology
  • HepG2-C3A cells

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    Cite this

    Wrzesinski, K., Magnone, M. C., Hansen, L. V., Ehrhorn Kruse, M., Bergauer, T., Bobadilla, M., Gubler, M., Mizrahi, J., Zhang, K., Andreasen, C. M., Joensen, K., Andersen, S. M., Olesen, J. B., Schaffalitzky de Muckadell, O. B., & Fey, S. J. (2013). HepG2/C3A 3D spheroids exhibit stable physiological functionality for at least 24 days after recovering from trypsinisation. Toxicology Research, 2(3), 163-172. https://doi.org/10.1039/c3tx20086h