Helium Ion Microscopy for the imaging of biological samples

Research output: Contribution to conference without publisher/journalPosterResearch

Abstract

Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.
Original languageEnglish
Publication date11. Jun 2018
Publication statusPublished - 11. Jun 2018
Event2nd international HeFIB conference on Helium and emerging Focused Ion Beams - Salzgasse, 4, 01067 , Dresden, Germany
Duration: 11. Jun 201813. Jun 2018
https://www.hzdr.de/db/Cms?pNid=3699

Conference

Conference2nd international HeFIB conference on Helium and emerging Focused Ion Beams
LocationSalzgasse, 4, 01067
CountryGermany
CityDresden
Period11/06/201813/06/2018
Internet address

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helium ions
microscopy
drying
critical point
ion microscopes
low currents
ion sources
embedding
helium
preparation
high resolution

Cite this

Leißner, T., Tavares, L., Gustafsson, L. R., Rubahn, H-G., Kjelstrup-Hansen, J., & Marcussen, N. (2018). Helium Ion Microscopy for the imaging of biological samples. Poster session presented at 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Germany.
Leißner, Till ; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter ; Kjelstrup-Hansen, Jakob ; Marcussen, Niels. / Helium Ion Microscopy for the imaging of biological samples. Poster session presented at 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Germany.
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title = "Helium Ion Microscopy for the imaging of biological samples",
abstract = "Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.",
author = "Till Lei{\ss}ner and Luciana Tavares and Gustafsson, {Linnea Rebecka} and Horst-G{\"u}nter Rubahn and Jakob Kjelstrup-Hansen and Niels Marcussen",
year = "2018",
month = "6",
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language = "English",
note = "2nd international HeFIB conference on Helium and emerging Focused Ion Beams, HeFIB 2018 ; Conference date: 11-06-2018 Through 13-06-2018",
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Leißner, T, Tavares, L, Gustafsson, LR, Rubahn, H-G, Kjelstrup-Hansen, J & Marcussen, N 2018, 'Helium Ion Microscopy for the imaging of biological samples', 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Germany, 11/06/2018 - 13/06/2018.

Helium Ion Microscopy for the imaging of biological samples. / Leißner, Till; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter; Kjelstrup-Hansen, Jakob; Marcussen, Niels.

2018. Poster session presented at 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Germany.

Research output: Contribution to conference without publisher/journalPosterResearch

TY - CONF

T1 - Helium Ion Microscopy for the imaging of biological samples

AU - Leißner, Till

AU - Tavares, Luciana

AU - Gustafsson, Linnea Rebecka

AU - Rubahn, Horst-Günter

AU - Kjelstrup-Hansen, Jakob

AU - Marcussen, Niels

PY - 2018/6/11

Y1 - 2018/6/11

N2 - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

AB - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

M3 - Poster

ER -

Leißner T, Tavares L, Gustafsson LR, Rubahn H-G, Kjelstrup-Hansen J, Marcussen N. Helium Ion Microscopy for the imaging of biological samples. 2018. Poster session presented at 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Germany.