Helium Ion Microscopy for the imaging of biological samples

Research output: Contribution to conference without publisher/journalPosterResearch

Abstract

Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.
Original languageEnglish
Publication date31. May 2018
Publication statusPublished - 31. May 2018
EventInternational Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018 - University of Southern Denmark, Sønderborg, Denmark
Duration: 30. May 20181. Jun 2018
https://www.sdu.dk/en/nibs2018

Conference

ConferenceInternational Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018
LocationUniversity of Southern Denmark
CountryDenmark
CitySønderborg
Period30/05/201801/06/2018
Internet address

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helium ions
microscopy
drying
critical point
ion microscopes
low currents
ion sources
embedding
helium
preparation
high resolution

Cite this

Leißner, T., Tavares, L., Gustafsson, L. R., Rubahn, H-G., Kjelstrup-Hansen, J., & Marcussen, N. (2018). Helium Ion Microscopy for the imaging of biological samples. Poster session presented at International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Denmark.
Leißner, Till ; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter ; Kjelstrup-Hansen, Jakob ; Marcussen, Niels. / Helium Ion Microscopy for the imaging of biological samples. Poster session presented at International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Denmark.
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title = "Helium Ion Microscopy for the imaging of biological samples",
abstract = "Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.",
author = "Till Lei{\ss}ner and Luciana Tavares and Gustafsson, {Linnea Rebecka} and Horst-G{\"u}nter Rubahn and Jakob Kjelstrup-Hansen and Niels Marcussen",
year = "2018",
month = "5",
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language = "English",
note = "null ; Conference date: 30-05-2018 Through 01-06-2018",
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Leißner, T, Tavares, L, Gustafsson, LR, Rubahn, H-G, Kjelstrup-Hansen, J & Marcussen, N 2018, 'Helium Ion Microscopy for the imaging of biological samples', International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Denmark, 30/05/2018 - 01/06/2018.

Helium Ion Microscopy for the imaging of biological samples. / Leißner, Till; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter; Kjelstrup-Hansen, Jakob; Marcussen, Niels.

2018. Poster session presented at International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Denmark.

Research output: Contribution to conference without publisher/journalPosterResearch

TY - CONF

T1 - Helium Ion Microscopy for the imaging of biological samples

AU - Leißner, Till

AU - Tavares, Luciana

AU - Gustafsson, Linnea Rebecka

AU - Rubahn, Horst-Günter

AU - Kjelstrup-Hansen, Jakob

AU - Marcussen, Niels

PY - 2018/5/31

Y1 - 2018/5/31

N2 - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

AB - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

M3 - Poster

ER -

Leißner T, Tavares L, Gustafsson LR, Rubahn H-G, Kjelstrup-Hansen J, Marcussen N. Helium Ion Microscopy for the imaging of biological samples. 2018. Poster session presented at International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Denmark.