Abstract

Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.
Original languageEnglish
Publication date31. May 2018
Publication statusPublished - 31. May 2018
EventInternational Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018 - University of Southern Denmark, Sønderborg, Denmark
Duration: 30. May 20181. Jun 2018
https://www.sdu.dk/en/nibs2018

Conference

ConferenceInternational Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018
LocationUniversity of Southern Denmark
CountryDenmark
CitySønderborg
Period30/05/201801/06/2018
Internet address

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