Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein

Marianne Jensby Nielsen, Steen Vang Petersen, Christian Jacobsen, Claus Oxvig, David Rees, Holger Jon Møller, Søren Kragh Moestrup

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Haptoglobin-related protein (Hpr) is a primate-specific plasma protein associated with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL) particles shown to be a part of the innate immune defense. Despite the assumption hitherto that Hpr does not bind to hemoglobin, the present study revealed that recombinant Hpr binds hemoglobin as efficiently as haptoglobin (Hp). However, in contrast to Hp, Hpr did not promote any high-affinity binding to the scavenger receptor CD163. Binding of hemoglobin to circulating native Hpr incorporated into the HDL fraction was indicated by hemoglobin-affinity precipitation of plasma Hpr together with apoL-I. In conclusion, plasma has 2 high-affinity hemoglobin-binding haptoglobins instead of one, but only Hp-hemoglobin complexes are efficiently recognized by CD163. Circulating Hpr-bound hemoglobin should therefore be taken into consideration when measuring "free" plasma hemoglobin. Furthermore, Hpr-bound hemoglobin might contribute to the biologic activity of the circulating apoL-I/Hpr-containing HDL particles.

Original languageEnglish
JournalBlood
Volume108
Issue number8
Pages (from-to)2846-9
Number of pages4
ISSN0006-4971
DOIs
Publication statusPublished - 2006

Fingerprint

Haptoglobins
Proteins
HDL Lipoproteins
Scavenger Receptors
Primates

Keywords

  • Amino Acid Sequence
  • Antigens, Neoplasm
  • Blood Proteins
  • Chromatography, Affinity
  • Haptoglobins
  • Hemoglobins
  • Humans
  • In Vitro Techniques
  • Protein Binding
  • Recombinant Proteins
  • Surface Plasmon Resonance

Cite this

Nielsen, M. J., Petersen, S. V., Jacobsen, C., Oxvig, C., Rees, D., Møller, H. J., & Moestrup, S. K. (2006). Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein. Blood, 108(8), 2846-9. https://doi.org/10.1182/blood-2006-05-022327
Nielsen, Marianne Jensby ; Petersen, Steen Vang ; Jacobsen, Christian ; Oxvig, Claus ; Rees, David ; Møller, Holger Jon ; Moestrup, Søren Kragh. / Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein. In: Blood. 2006 ; Vol. 108, No. 8. pp. 2846-9.
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abstract = "Haptoglobin-related protein (Hpr) is a primate-specific plasma protein associated with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL) particles shown to be a part of the innate immune defense. Despite the assumption hitherto that Hpr does not bind to hemoglobin, the present study revealed that recombinant Hpr binds hemoglobin as efficiently as haptoglobin (Hp). However, in contrast to Hp, Hpr did not promote any high-affinity binding to the scavenger receptor CD163. Binding of hemoglobin to circulating native Hpr incorporated into the HDL fraction was indicated by hemoglobin-affinity precipitation of plasma Hpr together with apoL-I. In conclusion, plasma has 2 high-affinity hemoglobin-binding haptoglobins instead of one, but only Hp-hemoglobin complexes are efficiently recognized by CD163. Circulating Hpr-bound hemoglobin should therefore be taken into consideration when measuring {"}free{"} plasma hemoglobin. Furthermore, Hpr-bound hemoglobin might contribute to the biologic activity of the circulating apoL-I/Hpr-containing HDL particles.",
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Nielsen, MJ, Petersen, SV, Jacobsen, C, Oxvig, C, Rees, D, Møller, HJ & Moestrup, SK 2006, 'Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein', Blood, vol. 108, no. 8, pp. 2846-9. https://doi.org/10.1182/blood-2006-05-022327

Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein. / Nielsen, Marianne Jensby; Petersen, Steen Vang; Jacobsen, Christian; Oxvig, Claus; Rees, David; Møller, Holger Jon; Moestrup, Søren Kragh.

In: Blood, Vol. 108, No. 8, 2006, p. 2846-9.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein

AU - Nielsen, Marianne Jensby

AU - Petersen, Steen Vang

AU - Jacobsen, Christian

AU - Oxvig, Claus

AU - Rees, David

AU - Møller, Holger Jon

AU - Moestrup, Søren Kragh

PY - 2006

Y1 - 2006

N2 - Haptoglobin-related protein (Hpr) is a primate-specific plasma protein associated with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL) particles shown to be a part of the innate immune defense. Despite the assumption hitherto that Hpr does not bind to hemoglobin, the present study revealed that recombinant Hpr binds hemoglobin as efficiently as haptoglobin (Hp). However, in contrast to Hp, Hpr did not promote any high-affinity binding to the scavenger receptor CD163. Binding of hemoglobin to circulating native Hpr incorporated into the HDL fraction was indicated by hemoglobin-affinity precipitation of plasma Hpr together with apoL-I. In conclusion, plasma has 2 high-affinity hemoglobin-binding haptoglobins instead of one, but only Hp-hemoglobin complexes are efficiently recognized by CD163. Circulating Hpr-bound hemoglobin should therefore be taken into consideration when measuring "free" plasma hemoglobin. Furthermore, Hpr-bound hemoglobin might contribute to the biologic activity of the circulating apoL-I/Hpr-containing HDL particles.

AB - Haptoglobin-related protein (Hpr) is a primate-specific plasma protein associated with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL) particles shown to be a part of the innate immune defense. Despite the assumption hitherto that Hpr does not bind to hemoglobin, the present study revealed that recombinant Hpr binds hemoglobin as efficiently as haptoglobin (Hp). However, in contrast to Hp, Hpr did not promote any high-affinity binding to the scavenger receptor CD163. Binding of hemoglobin to circulating native Hpr incorporated into the HDL fraction was indicated by hemoglobin-affinity precipitation of plasma Hpr together with apoL-I. In conclusion, plasma has 2 high-affinity hemoglobin-binding haptoglobins instead of one, but only Hp-hemoglobin complexes are efficiently recognized by CD163. Circulating Hpr-bound hemoglobin should therefore be taken into consideration when measuring "free" plasma hemoglobin. Furthermore, Hpr-bound hemoglobin might contribute to the biologic activity of the circulating apoL-I/Hpr-containing HDL particles.

KW - Amino Acid Sequence

KW - Antigens, Neoplasm

KW - Blood Proteins

KW - Chromatography, Affinity

KW - Haptoglobins

KW - Hemoglobins

KW - Humans

KW - In Vitro Techniques

KW - Protein Binding

KW - Recombinant Proteins

KW - Surface Plasmon Resonance

U2 - 10.1182/blood-2006-05-022327

DO - 10.1182/blood-2006-05-022327

M3 - Journal article

VL - 108

SP - 2846

EP - 2849

JO - Blood

JF - Blood

SN - 0006-4971

IS - 8

ER -

Nielsen MJ, Petersen SV, Jacobsen C, Oxvig C, Rees D, Møller HJ et al. Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein. Blood. 2006;108(8):2846-9. https://doi.org/10.1182/blood-2006-05-022327