Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky; Kotb Abdelmohsen; Akihisa Abe; Md Joynal Abedin; Hagai Abeliovich; Abraham Acevedo Arozena; Hiroaki Adachi; Christopher M. Adams; Peter D. Adams; Khosrow Adeli; Peter J. Adhihetty; Sharon G. Adler; Galila Agam; Rajesh Agarwal; Manish K. Aghi; Maria Agnello; Patrizia Agostinis; Patricia V. Aguilar; Julio Aguirre-Ghiso; Edoardo M. Airoldi; Slimane Ait-Si-Ali; Takahiko Akematsu; Emmanuel T. Akporiaye; Mohamed Al-Rubeai; Guillermo M. Albaiceta; Chris Albanese; Diego Albani; Matthew L. Albert; Jesus Aldudo; Hana Algül; Mehrdad Alirezaei; Iraide Alloza; Alexandru Almasan; Maylin Almonte-Beceril; Emad S. Alnemri; Covadonga Alonso; Nihal Altan-Bonnet; Dario C. Altieri; Silvia Alvarez; Lydia Alvarez-Erviti; Sandro Alves; Giuseppina Amadoro; Atsuo Amano; Nils J. Færgeman; Barbara Guerra; Ying Jan Wang

doi:10.1080/15548627.2015.1100356

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky^*
, Kotb Abdelmohsen
, Akihisa Abe
, Md Joynal Abedin
, Hagai Abeliovich
, Abraham Acevedo Arozena
, Hiroaki Adachi
, Christopher M. Adams
, Peter D. Adams
, Khosrow Adeli
, Peter J. Adhihetty
, Sharon G. Adler
, Galila Agam
, Rajesh Agarwal
, Manish K. Aghi
, Maria Agnello
, Patrizia Agostinis
, Patricia V. Aguilar
, Julio Aguirre-Ghiso
, Edoardo M. Airoldi

Slimane Ait-Si-Ali, Takahiko Akematsu, Emmanuel T. Akporiaye, Mohamed Al-Rubeai, Guillermo M. Albaiceta, Chris Albanese, Diego Albani, Matthew L. Albert, Jesus Aldudo, Hana Algül, Mehrdad Alirezaei, Iraide Alloza, Alexandru Almasan, Maylin Almonte-Beceril, Emad S. Alnemri, Covadonga Alonso, Nihal Altan-Bonnet, Dario C. Altieri, Silvia Alvarez, Lydia Alvarez-Erviti, Sandro Alves, Giuseppina Amadoro, Atsuo Amano, Nils J. Færgeman, Barbara Guerra, Ying Jan Wang

^*Corresponding author for this work

University of Michigan
National Institute on Aging
Tokyo Medical University
University of Minnesota
The Hebrew University of Jerusalem
MRC Harwell
University of Occupational and Environmental Health Japan
Iowa State University
University of Glasgow
The Hospital for Sick Children
University of Florida
Los Angeles Biomedical Research Institute
Ben-Gurion University of the Negev
University of California
University of Palermo
University of Texas at Austin
Icahn School of Medicine at Mount Sinai
Broad Institute
Harvard University
Paris Hospitals and Paris Descartes University
University of Vienna
Sidra Medical and Research Center
University College Dublin
University of Oviedo
Georgetown University
Mario Negri Institute for Pharmacological Research
Institut Pasteur
The University Hospital Klinikum rechts der Isar
Scripps Research
María Díaz de Haro
Materials Physics Center MPC
Cleveland Clinic Foundation
Thomas Jefferson University
National Heart, Lung, and Blood Institute
The Wistar Institute
University of Buenos Aires
National Research Council
Osaka University

Research output: Contribution to journal › Editorial › peer-review

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Original language	English
Journal	Autophagy
Volume	12
Issue number	1
Pages (from-to)	1-222
Number of pages	222
ISSN	1554-8627
DOIs	https://doi.org/10.1080/15548627.2015.1100356
Publication status	Published - 21. Jan 2016

Funding

This work was supported in part by the National Institutes of Health, including Public Health Service grant GM053396 to D.J.K. Due to space and other limitations, it is not possible to include all other sources of financial support.

Keywords

Autolysosome
Autophagosome
Chaperonemediated autophagy
Flux
LC3
Lysosome
Macroautophagy
Phagophore
Stress
Vacuole
Humans
Biological Assay/methods
Animals
Autophagy/physiology
Computer Simulation

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10.1080/15548627.2015.1100356

kaup_12_01_1100356Accepted manuscript, 8.91 MB

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Cite this

Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., Adhihetty, P. J., Adler, S. G., Agam, G., Agarwal, R., Aghi, M. K., Agnello, M., Agostinis, P., Aguilar, P. V., Aguirre-Ghiso, J., ... Wang, Y. J. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222. https://doi.org/10.1080/15548627.2015.1100356

@article{a252dec82aa846a1822b505307e2e693,

title = "Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)",

keywords = "Autolysosome, Autophagosome, Chaperonemediated autophagy, Flux, LC3, Lysosome, Macroautophagy, Phagophore, Stress, Vacuole, Humans, Biological Assay/methods, Animals, Autophagy/physiology, Computer Simulation",

author = "Klionsky, \{Daniel J.\} and Kotb Abdelmohsen and Akihisa Abe and Abedin, \{Md Joynal\} and Hagai Abeliovich and Arozena, \{Abraham Acevedo\} and Hiroaki Adachi and Adams, \{Christopher M.\} and Adams, \{Peter D.\} and Khosrow Adeli and Adhihetty, \{Peter J.\} and Adler, \{Sharon G.\} and Galila Agam and Rajesh Agarwal and Aghi, \{Manish K.\} and Maria Agnello and Patrizia Agostinis and Aguilar, \{Patricia V.\} and Julio Aguirre-Ghiso and Airoldi, \{Edoardo M.\} and Slimane Ait-Si-Ali and Takahiko Akematsu and Akporiaye, \{Emmanuel T.\} and Mohamed Al-Rubeai and Albaiceta, \{Guillermo M.\} and Chris Albanese and Diego Albani and Albert, \{Matthew L.\} and Jesus Aldudo and Hana Alg{\"u}l and Mehrdad Alirezaei and Iraide Alloza and Alexandru Almasan and Maylin Almonte-Beceril and Alnemri, \{Emad S.\} and Covadonga Alonso and Nihal Altan-Bonnet and Altieri, \{Dario C.\} and Silvia Alvarez and Lydia Alvarez-Erviti and Sandro Alves and Giuseppina Amadoro and Atsuo Amano and F{\ae}rgeman, \{Nils J.\} and Barbara Guerra and Wang, \{Ying Jan\}",

year = "2016",

month = jan,

day = "21",

doi = "10.1080/15548627.2015.1100356",

language = "English",

volume = "12",

pages = "1--222",

journal = "Autophagy",

issn = "1554-8627",

publisher = "Taylor \& Francis",

number = "1",

}

Klionsky, DJ, Abdelmohsen, K, Abe, A, Abedin, MJ, Abeliovich, H, Arozena, AA, Adachi, H, Adams, CM, Adams, PD, Adeli, K, Adhihetty, PJ, Adler, SG, Agam, G, Agarwal, R, Aghi, MK, Agnello, M, Agostinis, P, Aguilar, PV, Aguirre-Ghiso, J, Airoldi, EM, Ait-Si-Ali, S, Akematsu, T, Akporiaye, ET, Al-Rubeai, M, Albaiceta, GM, Albanese, C, Albani, D, Albert, ML, Aldudo, J, Algül, H, Alirezaei, M, Alloza, I, Almasan, A, Almonte-Beceril, M, Alnemri, ES, Alonso, C, Altan-Bonnet, N, Altieri, DC, Alvarez, S, Alvarez-Erviti, L, Alves, S, Amadoro, G, Amano, A, Færgeman, NJ , Guerra, B & Wang, YJ 2016, 'Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)', Autophagy, vol. 12, no. 1, pp. 1-222. https://doi.org/10.1080/15548627.2015.1100356

TY - JOUR

T1 - Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

AU - Klionsky, Daniel J.

AU - Abdelmohsen, Kotb

AU - Abe, Akihisa

AU - Abedin, Md Joynal

AU - Abeliovich, Hagai

AU - Arozena, Abraham Acevedo

AU - Adachi, Hiroaki

AU - Adams, Christopher M.

AU - Adams, Peter D.

AU - Adeli, Khosrow

AU - Adhihetty, Peter J.

AU - Adler, Sharon G.

AU - Agam, Galila

AU - Agarwal, Rajesh

AU - Aghi, Manish K.

AU - Agnello, Maria

AU - Agostinis, Patrizia

AU - Aguilar, Patricia V.

AU - Aguirre-Ghiso, Julio

AU - Airoldi, Edoardo M.

AU - Ait-Si-Ali, Slimane

AU - Akematsu, Takahiko

AU - Akporiaye, Emmanuel T.

AU - Al-Rubeai, Mohamed

AU - Albaiceta, Guillermo M.

AU - Albanese, Chris

AU - Albani, Diego

AU - Albert, Matthew L.

AU - Aldudo, Jesus

AU - Algül, Hana

AU - Alirezaei, Mehrdad

AU - Alloza, Iraide

AU - Almasan, Alexandru

AU - Almonte-Beceril, Maylin

AU - Alnemri, Emad S.

AU - Alonso, Covadonga

AU - Altan-Bonnet, Nihal

AU - Altieri, Dario C.

AU - Alvarez, Silvia

AU - Alvarez-Erviti, Lydia

AU - Alves, Sandro

AU - Amadoro, Giuseppina

AU - Amano, Atsuo

AU - Færgeman, Nils J.

AU - Guerra, Barbara

AU - Wang, Ying Jan

PY - 2016/1/21

Y1 - 2016/1/21

KW - Autolysosome

KW - Autophagosome

KW - Chaperonemediated autophagy

KW - Flux

KW - LC3

KW - Lysosome

KW - Macroautophagy

KW - Phagophore

KW - Stress

KW - Vacuole

KW - Humans

KW - Biological Assay/methods

KW - Animals

KW - Autophagy/physiology

KW - Computer Simulation

U2 - 10.1080/15548627.2015.1100356

DO - 10.1080/15548627.2015.1100356

M3 - Editorial

C2 - 26799652

AN - SCOPUS:85013763791

SN - 1554-8627

VL - 12

SP - 1

EP - 222

JO - Autophagy

JF - Autophagy

IS - 1

ER -