GPC-modalities for Neurophotonics and Optogenetics

Jesper Glückstad; René L. Eriksen; Andreas Gejl Madsen

doi:10.1117/12.2583507

GPC-modalities for Neurophotonics and Optogenetics

Jesper Glückstad^*, René L. Eriksen, Andreas Gejl Madsen

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

The powerful discipline of optogenetics can be described as “the branch of biotechnology which combines genetic engineering with optics to observe and control the function of genetically targeted groups of cells with light”. Using advanced laser beam-shaping such as Generalized Phase Contrast (GPC) and derived holographic modalities makes it possible to take advantage of cutting-edge two-photon technology to develop an unprecedented ‘circuit optogenetics’ platform with both high spatio-temporal selectivity and high penetration depth without disturbing speckle-noise. We will report on the latest laser shaping GPC-modalities with temporal focusing to uniquely sculpt patterns of one- and two-photon excitations for living neural-circuits and genetically modified light-sensitive cells.

Original language	English
Title of host publication	Complex Light and Optical Forces XV
Editors	Enrique J. Galvez, Halina Rubinsztein-Dunlop, David L. Andrews
Number of pages	7
Volume	11701
Publisher	SPIE - International Society for Optical Engineering
Publication date	2021
ISBN (Electronic)	9781510642379
DOIs	https://doi.org/10.1117/12.2583507
Publication status	Published - 2021
Event	Spie Opto - Online, California, United States Duration: 6. Mar 2021 → 12. Mar 2021

Conference

Conference	Spie Opto
Country/Territory	United States
City	Online, California
Period	06/03/2021 → 12/03/2021

Keywords

Generalized Phase Contrast (GPC)
Neurophotonics
Optogenetics

Documents & Links

10.1117/12.2583507

Open access versionFinal published version, 3.52 MB

Cite this

@inproceedings{00761508f22047e981939cd85dcf8c42,

title = "GPC-modalities for Neurophotonics and Optogenetics",

abstract = "The powerful discipline of optogenetics can be described as “the branch of biotechnology which combines genetic engineering with optics to observe and control the function of genetically targeted groups of cells with light”. Using advanced laser beam-shaping such as Generalized Phase Contrast (GPC) and derived holographic modalities makes it possible to take advantage of cutting-edge two-photon technology to develop an unprecedented {\textquoteleft}circuit optogenetics{\textquoteright} platform with both high spatio-temporal selectivity and high penetration depth without disturbing speckle-noise. We will report on the latest laser shaping GPC-modalities with temporal focusing to uniquely sculpt patterns of one- and two-photon excitations for living neural-circuits and genetically modified light-sensitive cells. ",

keywords = "Generalized Phase Contrast (GPC), Neurophotonics, Optogenetics",

author = "Jesper Gl{\"u}ckstad and Eriksen, {Ren{\'e} L.} and Madsen, {Andreas Gejl}",

year = "2021",

doi = "10.1117/12.2583507",

language = "English",

volume = "11701",

editor = "Galvez, {Enrique J.} and Rubinsztein-Dunlop,, {Halina } and Andrews, {David L. }",

booktitle = "Complex Light and Optical Forces XV",

publisher = "SPIE - International Society for Optical Engineering",

address = "United States",

note = "Spie Opto ; Conference date: 06-03-2021 Through 12-03-2021",

}

GPC-modalities for Neurophotonics and Optogenetics. / Glückstad, Jesper ; Eriksen, René L.; Madsen, Andreas Gejl.

Complex Light and Optical Forces XV. ed. / Enrique J. Galvez; Halina Rubinsztein-Dunlop,; David L. Andrews. Vol. 11701 SPIE - International Society for Optical Engineering, 2021.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - GPC-modalities for Neurophotonics and Optogenetics

AU - Glückstad, Jesper

AU - Eriksen, René L.

AU - Madsen, Andreas Gejl

PY - 2021

Y1 - 2021

N2 - The powerful discipline of optogenetics can be described as “the branch of biotechnology which combines genetic engineering with optics to observe and control the function of genetically targeted groups of cells with light”. Using advanced laser beam-shaping such as Generalized Phase Contrast (GPC) and derived holographic modalities makes it possible to take advantage of cutting-edge two-photon technology to develop an unprecedented ‘circuit optogenetics’ platform with both high spatio-temporal selectivity and high penetration depth without disturbing speckle-noise. We will report on the latest laser shaping GPC-modalities with temporal focusing to uniquely sculpt patterns of one- and two-photon excitations for living neural-circuits and genetically modified light-sensitive cells.

AB - The powerful discipline of optogenetics can be described as “the branch of biotechnology which combines genetic engineering with optics to observe and control the function of genetically targeted groups of cells with light”. Using advanced laser beam-shaping such as Generalized Phase Contrast (GPC) and derived holographic modalities makes it possible to take advantage of cutting-edge two-photon technology to develop an unprecedented ‘circuit optogenetics’ platform with both high spatio-temporal selectivity and high penetration depth without disturbing speckle-noise. We will report on the latest laser shaping GPC-modalities with temporal focusing to uniquely sculpt patterns of one- and two-photon excitations for living neural-circuits and genetically modified light-sensitive cells.

KW - Generalized Phase Contrast (GPC)

KW - Neurophotonics

KW - Optogenetics

U2 - 10.1117/12.2583507

DO - 10.1117/12.2583507

M3 - Article in proceedings

VL - 11701

BT - Complex Light and Optical Forces XV

A2 - Galvez, Enrique J.

A2 - Rubinsztein-Dunlop,, Halina

A2 - Andrews, David L.

PB - SPIE - International Society for Optical Engineering

T2 - Spie Opto

Y2 - 6 March 2021 through 12 March 2021

ER -

GPC-modalities for Neurophotonics and Optogenetics

Abstract

Conference

Keywords

Documents & Links

Fingerprint

Cite this