Glycolytic oscillations and intracellular K+ concentration are strongly coupled in the yeast Saccharomyces cerevisiae

Lars F. Olsen, Roberto P. Stock, L. A. Bagatolli*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We measured temporal oscillations of intracellular K+ concentration in yeast cells exhibiting glycolytic oscillations using fluorescence spectroscopy and microscopy methods. These oscillations showed the same period as those of glycolytic metabolites (NADH, ATP), indicating a strong coupling between them. We experimentally ruled out that oscillations originate in extra- or intracellular K+ fluxes and conclude that these oscillations arise from fluctuations in free and adsorbed states of K+ in the cell interior. Oscillations in K+ showed a strong dependence on ATP and the organization of the cell cytoskeleton. Our results challenge the widely held view that intracellular K+ predominantly exists in a free state. They can, however, be productively understood in terms of Gilbert Ling's Association-Induction hypothesis.

Original languageEnglish
Article number108257
JournalArchives of Biochemistry and Biophysics
Volume681
ISSN0003-9861
DOIs
Publication statusPublished - 15. Mar 2020

Fingerprint

Yeast
Saccharomyces cerevisiae
Adenosine Triphosphate
Fluorescence microscopy
Fluorescence spectroscopy
Metabolites
NAD
Cells
Fluxes
Fluorescence Microscopy

Keywords

  • Association-induction hypothesis
  • Glycolytic oscillations
  • Intracellular potassium concentration
  • PBFI fluorescence
  • Yeast cytosol

Cite this

@article{cc2d7e0be2a14a1982b07b25ad13e0ed,
title = "Glycolytic oscillations and intracellular K+ concentration are strongly coupled in the yeast Saccharomyces cerevisiae",
abstract = "We measured temporal oscillations of intracellular K+ concentration in yeast cells exhibiting glycolytic oscillations using fluorescence spectroscopy and microscopy methods. These oscillations showed the same period as those of glycolytic metabolites (NADH, ATP), indicating a strong coupling between them. We experimentally ruled out that oscillations originate in extra- or intracellular K+ fluxes and conclude that these oscillations arise from fluctuations in free and adsorbed states of K+ in the cell interior. Oscillations in K+ showed a strong dependence on ATP and the organization of the cell cytoskeleton. Our results challenge the widely held view that intracellular K+ predominantly exists in a free state. They can, however, be productively understood in terms of Gilbert Ling's Association-Induction hypothesis.",
keywords = "Association-induction hypothesis, Glycolytic oscillations, Intracellular potassium concentration, PBFI fluorescence, Yeast cytosol",
author = "Olsen, {Lars F.} and Stock, {Roberto P.} and Bagatolli, {L. A.}",
year = "2020",
month = "3",
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doi = "10.1016/j.abb.2020.108257",
language = "English",
volume = "681",
journal = "Archives of Biochemistry and Biophysics",
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Glycolytic oscillations and intracellular K+ concentration are strongly coupled in the yeast Saccharomyces cerevisiae. / Olsen, Lars F.; Stock, Roberto P.; Bagatolli, L. A.

In: Archives of Biochemistry and Biophysics, Vol. 681, 108257, 15.03.2020.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Glycolytic oscillations and intracellular K+ concentration are strongly coupled in the yeast Saccharomyces cerevisiae

AU - Olsen, Lars F.

AU - Stock, Roberto P.

AU - Bagatolli, L. A.

PY - 2020/3/15

Y1 - 2020/3/15

N2 - We measured temporal oscillations of intracellular K+ concentration in yeast cells exhibiting glycolytic oscillations using fluorescence spectroscopy and microscopy methods. These oscillations showed the same period as those of glycolytic metabolites (NADH, ATP), indicating a strong coupling between them. We experimentally ruled out that oscillations originate in extra- or intracellular K+ fluxes and conclude that these oscillations arise from fluctuations in free and adsorbed states of K+ in the cell interior. Oscillations in K+ showed a strong dependence on ATP and the organization of the cell cytoskeleton. Our results challenge the widely held view that intracellular K+ predominantly exists in a free state. They can, however, be productively understood in terms of Gilbert Ling's Association-Induction hypothesis.

AB - We measured temporal oscillations of intracellular K+ concentration in yeast cells exhibiting glycolytic oscillations using fluorescence spectroscopy and microscopy methods. These oscillations showed the same period as those of glycolytic metabolites (NADH, ATP), indicating a strong coupling between them. We experimentally ruled out that oscillations originate in extra- or intracellular K+ fluxes and conclude that these oscillations arise from fluctuations in free and adsorbed states of K+ in the cell interior. Oscillations in K+ showed a strong dependence on ATP and the organization of the cell cytoskeleton. Our results challenge the widely held view that intracellular K+ predominantly exists in a free state. They can, however, be productively understood in terms of Gilbert Ling's Association-Induction hypothesis.

KW - Association-induction hypothesis

KW - Glycolytic oscillations

KW - Intracellular potassium concentration

KW - PBFI fluorescence

KW - Yeast cytosol

U2 - 10.1016/j.abb.2020.108257

DO - 10.1016/j.abb.2020.108257

M3 - Journal article

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AN - SCOPUS:85077650633

VL - 681

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

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