Global mapping of protein phosphorylation events identifies novel signalling hubs mediating fatty acid starvation responses in Saccharomyces cerevisiae

Dennis Pultz, Martin Bennetzen, Steven Vestergaard Rødkær, Adam Stefanko, Jens S. Andersen, Christer S. Ejsing, Nils J. Færgeman

Research output: Contribution to conference without publisher/journalPosterCommunication

Abstract

Dietary restriction (DR) extends the life span of multiple species, ranging from single-celled organisms like yeast to mammals. This increase in longevity by dietary restriction is coupled to profound beneficial effects on age-related pathology. Despite the number of studies on DR and the physiological changes DR induces, only little is known about the genetics and signalling networks, which regulate the DR response. We have recently shown that inhibition of fatty acid synthesis in Saccharomyces cerevisiae induces autophagy mediated by TORC1 signalling and affects life span. In the present study, we have used quantitative mass spectrometry to further examine how inhibition of fatty acid synthesis affects cellular signalling events in Saccharomyces cerevisiae. We have identified approximately 2000 phosphorylation sites of which more than 400 have been identified as being regulated in a temporal manner in response to inhibition of fatty acid synthesis by cerulenin. By in silico analysis of these phosphorylation events, we have identified the major downstream regulated processes and signalling networks mediating the cellular response to fatty acid starvation. The analysis further identifies putative signalling components, which confer cerulenin-induced changes in the cellular lipidome.
Original languageEnglish
Publication date2011
Publication statusPublished - 2011

Cite this

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title = "Global mapping of protein phosphorylation events identifies novel signalling hubs mediating fatty acid starvation responses in Saccharomyces cerevisiae",
abstract = "Dietary restriction (DR) extends the life span of multiple species, ranging from single-celled organisms like yeast to mammals. This increase in longevity by dietary restriction is coupled to profound beneficial effects on age-related pathology. Despite the number of studies on DR and the physiological changes DR induces, only little is known about the genetics and signalling networks, which regulate the DR response. We have recently shown that inhibition of fatty acid synthesis in Saccharomyces cerevisiae induces autophagy mediated by TORC1 signalling and affects life span. In the present study, we have used quantitative mass spectrometry to further examine how inhibition of fatty acid synthesis affects cellular signalling events in Saccharomyces cerevisiae. We have identified approximately 2000 phosphorylation sites of which more than 400 have been identified as being regulated in a temporal manner in response to inhibition of fatty acid synthesis by cerulenin. By in silico analysis of these phosphorylation events, we have identified the major downstream regulated processes and signalling networks mediating the cellular response to fatty acid starvation. The analysis further identifies putative signalling components, which confer cerulenin-induced changes in the cellular lipidome.",
author = "Dennis Pultz and Martin Bennetzen and R{\o}dk{\ae}r, {Steven Vestergaard} and Adam Stefanko and Andersen, {Jens S.} and Ejsing, {Christer S.} and F{\ae}rgeman, {Nils J.}",
year = "2011",
language = "English",

}

Global mapping of protein phosphorylation events identifies novel signalling hubs mediating fatty acid starvation responses in Saccharomyces cerevisiae. / Pultz, Dennis; Bennetzen, Martin; Rødkær, Steven Vestergaard ; Stefanko, Adam; Andersen, Jens S.; Ejsing, Christer S.; Færgeman, Nils J.

2011.

Research output: Contribution to conference without publisher/journalPosterCommunication

TY - CONF

T1 - Global mapping of protein phosphorylation events identifies novel signalling hubs mediating fatty acid starvation responses in Saccharomyces cerevisiae

AU - Pultz, Dennis

AU - Bennetzen, Martin

AU - Rødkær, Steven Vestergaard

AU - Stefanko, Adam

AU - Andersen, Jens S.

AU - Ejsing, Christer S.

AU - Færgeman, Nils J.

PY - 2011

Y1 - 2011

N2 - Dietary restriction (DR) extends the life span of multiple species, ranging from single-celled organisms like yeast to mammals. This increase in longevity by dietary restriction is coupled to profound beneficial effects on age-related pathology. Despite the number of studies on DR and the physiological changes DR induces, only little is known about the genetics and signalling networks, which regulate the DR response. We have recently shown that inhibition of fatty acid synthesis in Saccharomyces cerevisiae induces autophagy mediated by TORC1 signalling and affects life span. In the present study, we have used quantitative mass spectrometry to further examine how inhibition of fatty acid synthesis affects cellular signalling events in Saccharomyces cerevisiae. We have identified approximately 2000 phosphorylation sites of which more than 400 have been identified as being regulated in a temporal manner in response to inhibition of fatty acid synthesis by cerulenin. By in silico analysis of these phosphorylation events, we have identified the major downstream regulated processes and signalling networks mediating the cellular response to fatty acid starvation. The analysis further identifies putative signalling components, which confer cerulenin-induced changes in the cellular lipidome.

AB - Dietary restriction (DR) extends the life span of multiple species, ranging from single-celled organisms like yeast to mammals. This increase in longevity by dietary restriction is coupled to profound beneficial effects on age-related pathology. Despite the number of studies on DR and the physiological changes DR induces, only little is known about the genetics and signalling networks, which regulate the DR response. We have recently shown that inhibition of fatty acid synthesis in Saccharomyces cerevisiae induces autophagy mediated by TORC1 signalling and affects life span. In the present study, we have used quantitative mass spectrometry to further examine how inhibition of fatty acid synthesis affects cellular signalling events in Saccharomyces cerevisiae. We have identified approximately 2000 phosphorylation sites of which more than 400 have been identified as being regulated in a temporal manner in response to inhibition of fatty acid synthesis by cerulenin. By in silico analysis of these phosphorylation events, we have identified the major downstream regulated processes and signalling networks mediating the cellular response to fatty acid starvation. The analysis further identifies putative signalling components, which confer cerulenin-induced changes in the cellular lipidome.

M3 - Poster

ER -