Glial membranes at the node of Ranvier prevent neurite outgrowth

Jeffrey K Huang, Greg R Phillips, Alejandro D Roth, Liliana Pedraza, Weisong Shan, Wiam Belkaid, Sha Mi, Åsa Fex Svenningsen, Laurence Florens, John R Yates, David R Colman

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) of mammals restricted neurite outgrowth of cultured neurons. Proteomic analysis of these membranes revealed several inhibitors of neurite outgrowth, including the oligodendrocyte myelin glycoprotein (OMgp). In rat spinal cord, OMgp was not localized to compact myelin, as previously thought, but to oligodendroglia-like cells, whose processes converge to form a ring that completely encircles the nodes. In OMgp-null mice, CNS nodes were abnormally wide and collateral sprouting was observed. Nodal ensheathment in the CNS may stabilize the node and prevent axonal sprouting.
Original languageEnglish
JournalScience
Volume310
Issue number5755
Pages (from-to)1813-7
Number of pages4
ISSN0036-8075
DOIs
Publication statusPublished - 2005

Keywords

  • Animals
  • Antigens
  • Axons
  • Cattle
  • Cell Surface Extensions
  • Cells, Cultured
  • Ganglia, Spinal
  • Humans
  • Mice
  • Myelin Sheath
  • Myelin-Associated Glycoprotein
  • Neurites
  • Neuroglia
  • Oligodendroglia
  • Proteoglycans
  • Proteomics
  • Ranvier's Nodes
  • Rats
  • Spinal Cord

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