Glass Microsphere-Supported Giant Vesicles for the Observation of Self-Reproduction of Lipid Boundaries

Michele Fiore*, Ofelia Maniti, Agnes Girard-Egrot, Pierre Alain Monnard, Peter Strazewski

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Growth and division experiments on phospholipid boundaries were carried out using glass microsphere-supported phospholipid (DOPC) giant vesicles (GVs) fed with a fatty acid solution (oleic acid) at two distinct feeding rates. Both fast and slow feeding methods produced daughter GVs. Under slow feeding conditions the membrane growth process (evagination, buds, filaments) was observed in detail by fluorescence microscopy. The density difference between supported mother vesicles and newly formed daughter vesicles allowed their easy separation. Mass spectrometric analysis of the resulting mother and daughter GVs showed that the composition of both vesicle types was a mixture of original supported phospholipids and added fatty acids reflecting the total composition of amphiphiles after the feeding process. Thus, self-reproduction of phospholipid vesicles can take place under preservation of the lipid composition but different aggregate size.

Original languageEnglish
JournalAngewandte Chemie - International Edition
Volume57
Issue number1
Pages (from-to)282-286
ISSN1433-7851
DOIs
Publication statusPublished - 2018

Keywords

  • epifluorescence microscopy
  • growth and division processes
  • lipid boundaries
  • supported giant vesicles

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