Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Morad Kamand; Mirolyuba Ilieva; Sheena Louise Forsberg; Mads Thomassen; Åsa Fex Svenningsen; Bjørn Holst; Morten Meyer; Tanja Maria Michel

doi:10.1016/j.scr.2020.101834

Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Morad Kamand, Mirolyuba Ilieva, Sheena Louise Forsberg, Mads Thomassen, Åsa Fex Svenningsen, Bjørn Holst, Morten Meyer, Tanja Maria Michel^*

^*Corresponding author for this work

Bioneer A/S

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.

Original language	English
Article number	101834
Journal	Stem Cell Research
Volume	46
Number of pages	4
ISSN	1873-5061
DOIs	https://doi.org/10.1016/j.scr.2020.101834
Publication status	Published - Jul 2020

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10.1016/j.scr.2020.101834Licence: CC BY-NC-ND

Open Access VersionFinal published version, 1.09 MBLicence: CC BY-NC-ND

1 Ph.D. thesis

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain
Kamand, M., 24. Jun 2022, Syddansk Universitet. Det Sundhedsvidenskabelige Fakultet. 225 p.
Research output: Thesis › Ph.D. thesis

Cite this

@article{b1651e5f246c4fc2b37174cb64310ea9,

title = "Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder",

abstract = "Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.",

author = "Morad Kamand and Mirolyuba Ilieva and Forsberg, {Sheena Louise} and Mads Thomassen and {Fex Svenningsen}, {\AA}sa and Bj{\o}rn Holst and Morten Meyer and Michel, {Tanja Maria}",

year = "2020",

month = jul,

doi = "10.1016/j.scr.2020.101834",

language = "English",

volume = "46",

journal = "Stem Cell Research",

issn = "1873-5061",

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T1 - Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

AU - Kamand, Morad

AU - Ilieva, Mirolyuba

AU - Forsberg, Sheena Louise

AU - Thomassen, Mads

AU - Fex Svenningsen, Åsa

AU - Holst, Bjørn

AU - Meyer, Morten

AU - Michel, Tanja Maria

PY - 2020/7

Y1 - 2020/7

N2 - Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.

AB - Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.

U2 - 10.1016/j.scr.2020.101834

DO - 10.1016/j.scr.2020.101834

M3 - Journal article

C2 - 32447258

AN - SCOPUS:85084788952

SN - 1873-5061

VL - 46

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101834

ER -

Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Abstract

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Related research output

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain

Cite this